We narrowed to 10,632 results for: t7

1. 30 pCOLI_S_MBP

   Plasmid

   #160172

   Purpose

   N-term MBP 3C cleavable Spec resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 26 pCOLI_S_HIS

   Plasmid

   #160168

   Purpose

   N-term 6xHis 3C cleavable Spec resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HOXC6 (human) HIS-tag pET

   Plasmid

   #8565

   Depositor

   Corey Largman

   Insert

   HOXC6 (HOXC6 Human)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   This protein starts at an internal Met initially …

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. FN 127

   Plasmid

   #50496

   Depositor

   Kenneth Yamada

   Insert

   fibronectin (FN1 Human)
   

   Expression

   Bacterial

   Mutation

   repeats 9 and 10; P1407S, H1408P, R1410D; PHSRN t…

   Promoter

   T7

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pRSETA_ionKE

   Plasmid

   #172925

   Purpose

   Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

   Depositor

   Arnold Boersma

   Article

   Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

   Insert

   IonicStrengthSensorKE
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4IA9

   Plasmid

   #51320

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   WDR5 (WDR5 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Truncation: See Comments

   Promoter

   T7

   Available Since

   Feb. 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. rFIXX-bp chain A (IRESneo)

   Plasmid

   #82450

   Purpose

   Chain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.

   Depositor

   Robert Peters

   Insert

   rFIXX-bp chain A
   

   Tags

   Polyhistidine

   Expression

   Bacterial and Mammalian

   Promoter

   T7 RNA polymerase promoter

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTalin2_R10

   Plasmid

   #111043

   Purpose

   Plasmid expresses the talin2 rod domain R10 a 5 helix bundle.

   Depositor

   Ben Goult

   Article

   Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

   Insert

   TLN2 (Tln2 Mouse)
   

   Tags

   His Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTalin2_R9

   Plasmid

   #111042

   Purpose

   Plasmid expresses the talin2 rod domain R9 a 5 helix bundle.

   Depositor

   Ben Goult

   Article

   Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

   Insert

   TLN2 (Tln2 Mouse)
   

   Tags

   His Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEDuet40

    Plasmid

    #46378

    Depositor

    Hideo Iwai

    Article

    Ellila et al FEBS Lett. 2011 Oct 10.

    Insert

    TvoVMA intein
    

    Tags

    GB1 and H6-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMMDuet19

    Plasmid

    #45594

    Depositor

    Hideo Iwai

    Article

    Ellila et al FEBS Lett. 2011 Oct 10.

    Insert

    NpuDnaB mini intein
    

    Tags

    GB1 and H6-GB1

    Expression

    Bacterial

    Mutation

    removal of endonuclease domain

    Promoter

    T7

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4PZI

    Plasmid

    #61955

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MLL4:JMC054-D08:C226821 (KMT2B Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. Human Phosphomevalonate kinase (PMK)

    Plasmid

    #52430

    Purpose

    bacterial expression of human Phosphomevalonate kinase (PMK)

    Depositor

    Henry Miziorko

    Article

    Herdendorf et al Biochemistry. 2006 Mar 14;45(10):3235-42

    Insert

    Phosphomevalonate kinase (PMVK Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pET22-Strep-TEV-Nsp10

    Plasmid

    #154958

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
    

    Tags

    Strep-TEV

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. 5WBN (HDAC6)

    Plasmid

    #101267

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HDAC6 (HDAC6 Human)
    

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET28b-Ava3856

    Plasmid

    #45742

    Depositor

    Christopher T Walsh, Emily Balskus

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3856 (Ava_3856 Anabaena variabilis)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pSADuet760

    Plasmid

    #45613

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    MjaTFIIB mini intein
    

    Tags

    GB1 and H6-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. 4L1M

    Plasmid

    #60086

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HERC2:YTC007-A11:C225804 (HERC2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. rFIXX-bp chain B (IRESpuro)

    Plasmid

    #82451

    Purpose

    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.

    Depositor

    Robert Peters

    Insert

    rFIXX-bp chain B
    

    Tags

    Biotin

    Expression

    Bacterial and Mammalian

    Promoter

    T7 RNA polymerase promoter

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. GB1-GyrA

    Plasmid

    #128738

    Purpose

    Expresses GB1 fused to GyrA intein at the C-terminal

    Depositor

    Sharon Rozovsky

    Article

    Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cb

    Insert

    GB1
    

    Tags

    GyrA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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