We narrowed to 4,639 results for: crispr c plasmids

Showing: 4351 - 4380 of 4639 results

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pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2

Plasmid

#204852

Purpose

AAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouse

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd6-nSaCas9KKH(D10A)-UGI

Use

AAV and CRISPR

Mutation

D10A, E782K, N968K, R1015H in SaCas9

Promoter

EFS, miniU6

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ284-v2

Plasmid

#138119

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a v2

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

D172R, N563R and K569R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ284-RVR

Plasmid

#138117

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N563R, K569V and N573R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ289-RVR

Plasmid

#138127

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

ErCas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

D513R, K519V and N523R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ284-v1

Plasmid

#138118

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R mutation, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a v1

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

D172R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(SOD1)

Plasmid

#109321

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (SOD1 Human)

Use

AAV

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ223-STU

Plasmid

#138109

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminator

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

dAsCas12a-SRDX

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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rTTA-gRNA-AAV

Plasmid

#213036

Purpose

This vector contains rTTA expressed under CMV promoter and gRNA expressed under U6 promoter

Depositor

Nadav Ahituv

Article

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

rTTA-T2A-mCherry

Use

AAV and CRISPR

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgCHMP1A

Plasmid

#169321

Purpose

Expresses CHMP1a targeting sgRNA in non-lentiviral BPK1520 plasmid

Depositor

Francisca Vazquez

Article

Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

Insert

sgCHMP1A

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgCHMP4B

Plasmid

#169322

Purpose

Expresses CHMP4B targeting sgRNA in non-lentiviral BPK1520 plasmid

Depositor

Francisca Vazquez

Article

Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

Insert

sgCHMP4B

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ287-RVR

Plasmid

#138124

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

BsCas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N512R, K518V and N522R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ285-RVR

Plasmid

#138121

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a with N512R, K518V and N522R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Lb5Cas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N512R, K518V and N522R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pABE8e-NG-403Q-Nterm-AAV

Plasmid

#194651

Purpose

N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

Depositor

David Liu

Article

Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

Insert

ABE8e-NG N-term

Use

AAV

Tags

BPNLS and NpuN spilt intein

Promoter

TNNT2

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-scFVDnmt3L_bGHpA

Plasmid

#177356

Purpose

AAV expression of scFV-fused C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)

Use

AAV

Tags

myc and scFV

Expression

Mammalian

Mutation

194–415 aa

Promoter

CMV promoter

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7

Plasmid

#226916

Purpose

Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSp Cas9C

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-hudTET1CD-T2A-GFP (PX458)

Plasmid

#129026

Purpose

inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-EGFP

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

Plasmid

#129028

Purpose

inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

Plasmid

#129048

Purpose

inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA8 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

Plasmid

#129041

Purpose

inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA1 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

Plasmid

#129036

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA8 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

Plasmid

#129053

Purpose

targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA1 (mouseTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

Plasmid

#129029

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA1 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

Plasmid

#206178

Purpose

Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

Depositor

Yuxuan Guo

Article

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Cre

Use

AAV

Promoter

cTnT

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-9

Plasmid

#129061

Purpose

targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting mouse TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA9 (mouseTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-10

Plasmid

#129062

Purpose

targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting mouse TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA10 (mouseTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-11

Plasmid

#129063

Purpose

targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting mouse TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA11 (mouseTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-12

Plasmid

#129064

Purpose

targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting mouse TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA12 (mouseTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-2

Plasmid

#129054

Purpose

targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting mouse TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA2 (mouseTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-3

Plasmid

#129055

Purpose

targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting mouse TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA3 (mouseTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-4

Plasmid

#129056

Purpose

targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting mouse TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA4 (mouseTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge