We narrowed to 24,491 results for: Spr;

1. pCXLE-dCas9GFP-shP53

   Plasmid

   #102906

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9GFP-shP53
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUDP003

   Plasmid

   #101164

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM-WingGFP-tan

   Plasmid

   #83473

   Purpose

   EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   WingGFP-tan
   

   Use

   CRISPR

   Expression

   Bacterial and Insect

   Promoter

   Hsp70 and enhancer from D. melanogaster yellow ge…

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ141C2.0

   Plasmid

   #99905

   Purpose

   Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU6

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAN-PBAD-sgRNA-scramble

   Plasmid

   #62285

   Purpose

   expression of scramble sgRNA from the arabinose-inducible promoter

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   scramble sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLdSaCN

   Plasmid

   #123261

   Purpose

   Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

   Insert

   gRNA and SaCas9
   

   Use

   CRISPR; Leishmania

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. D. vulgaris Cas7/pHMGWA

   Plasmid

   #81184

   Purpose

   Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Insert

   Cas7
   

   Use

   CRISPR

   Tags

   6xHis - MBP - TEV protease site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIBR005

   Plasmid

   #177668

   Purpose

   Constitutive expression of FnCas12a

   Depositor

   John van der Oost

   Article

   Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ptreCas9-mKate2ps-T1gRNA

   Plasmid

   #62715

   Purpose

   Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA

   Depositor

   Leonidas Bleris

   Article

   Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

   Insert

   Cas9–mKate2ps
   

   Expression

   Mammalian

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ292-D570A

    Plasmid

    #129679

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    D570A; AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX458_RXRB_1

    Plasmid

    #104050

    Purpose

    Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RXRB gRNA (RXRB Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. NAX_CAGGS_CAS9_m4_EYFP

    Plasmid

    #167866

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Zebrafish-gRNA-0007

    Plasmid

    #42247

    Purpose

    gRNA targeted to zebrafish gene th1

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-th1 (th Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

14. D. vulgaris Cas5c/pHMGWA

    Plasmid

    #81182

    Purpose

    Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas5c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. D. vulgaris Cas8c/pHMGWA

    Plasmid

    #81183

    Purpose

    Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas8c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIK198

    Plasmid

    #65629

    Purpose

    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBK31 pHomL-TDH3p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179015

    Purpose

    Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBM23-pyrF-NT

    Plasmid

    #174384

    Purpose

    pBM23 contained pyrF and mini-CRISPR.

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    pyrF-NT
    

    Use

    Template for cloning fragments for chromosomal in…

    Expression

    Bacterial

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBM23-pyrF-CS

    Plasmid

    #174396

    Purpose

    pBM23 contained pyrF and mini-CRISPR.

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    pyrF-CS
    

    Use

    Template for cloning fragments for chromosomal in…

    Expression

    Bacterial

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX458_TEAD3_2

    Plasmid

    #86338

    Purpose

    Encodes gRNA for 3' target of human TEAD3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against TEAD3 (TEAD3 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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