We narrowed to 2,596 results for: ubiquitin

1. pET15b p97-Strep-His E314Amb

   Plasmid

   #169021

   Purpose

   Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Use

   Tags

   His and SBP

   Expression

   Bacterial

   Mutation

   E314TAG

   Promoter

   T7

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221 Ub-NLRP1 S1213 P1214R

   Plasmid

   #166830

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   P1214R

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW_0005

   Plasmid

   #102837

   Purpose

   Binary vector containing Sr22 PI573523 allele driven by maize ubiquitin promoter, Sr22 terminator

   Depositor

   Brande Wulff

   Article

   Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

   Insert

   Sr22 PI573523 (NEWENTRY Synthetic)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Maize ubiquitin

   Available since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDUET-GST AO7RE Y165A

   Plasmid

   #139428

   Purpose

   GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed tyrosine 165 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDUET-GST AO7RE P199A

   Plasmid

   #139427

   Purpose

   GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed proline 199 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDUET-GST AO7RE I137A

   Plasmid

   #139319

   Purpose

   GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed isoleucine 137 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223 LC3B Q116P G120

   Plasmid

   #129291

   Purpose

   Gateway entry clone encoding human deconjugation-resistant LC3B

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Gateway entry vector / entry clone

   Tags

   Expression

   Mutation

   Changed Glutamine 116 to Proline. Deleted amino a…

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-HA-RNF68 [dRING]

   Plasmid

   #126549

   Purpose

   Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RNF68 (PCGF1 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   RING domain deletion mutant [dRING mutation is a …

   Promoter

   LTR

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)

   Plasmid

   #119318

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A, C840A) in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP III (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   C41A, C46A, C840A

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PHAGE-P CMVt N-HA hE6-AP I (G20V)

    Plasmid

    #119308

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (G20V) (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    G20V

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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