We narrowed to 18,353 results for: Tec

1. pDAC446

   Plasmid

   #195239

   Purpose

   Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-miR-155 toehold switch

   Plasmid

   #184755

   Purpose

   Expresses miR-155 sensor with GFP output

   Depositor

   Allen Liu

   Article

   Wang et al ACS Synth Biol. 2019 May 17;8(5):1079-10

   Insert

   Green Fluorescence Protein
   

   Use

   Tags

   miR-155 toehold

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM117

   Plasmid

   #127212

   Purpose

   BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBI-MCS-EYFP

   Plasmid

   #58855

   Purpose

   The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIG3 N

   Plasmid

   #132941

   Purpose

   MLV packaging plasmid for N-tropic MLV gag-pol expression

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   N-tropic MLV gag-pol
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJeM1

   Plasmid

   #135088

   Purpose

   rhamnose-inducible expression vector for replication in E. coli and P. putida

   Depositor

   Josef Altenbuchner

   Article

   Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

   Insert

   rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmRFP_a_tubulin_C1

   Plasmid

   #21040

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Use

   Tags

   pmRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pBT279

   Plasmid

   #122609

   Purpose

   express FERNY-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   FERNY-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. JME4472

   Plasmid

   #129658

   Purpose

   URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

    Plasmid

    #209296

    Purpose

    mitoBE expression vector, target mtRNR2 gene.

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

    Insert

    COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. P16ΔS:S10-(MCS)

    Plasmid

    #108182

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    P16ΔS

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-GAVPO

    Plasmid

    #159923

    Purpose

    GAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    GAVPO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT445

    Plasmid

    #139386

    Purpose

    aTC inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    aTC inducible Nanoluc expression
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    aTC inducible promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT444

    Plasmid

    #139385

    Purpose

    IPTG inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    IPTG inducible Nanoluc expression,
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    IPTG inducible promoter

    Available since

    July 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT128c

    Plasmid

    #138512

    Purpose

    P2

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    inteinC-dSpCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMRBAD-link-CGFP

    Plasmid

    #52733

    Purpose

    L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    link-CGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRE-ChrimsonR-mCherry

    Plasmid

    #92207

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    ChimsonR-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET11a-link-NGFP

    Plasmid

    #52732

    Purpose

    IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    link-NGFP
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKHH030

    Plasmid

    #89358

    Purpose

    hU6 sgRNA cloning vector for CDKO system

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRE3G-c-fos-iRFP-B3-repRNA-A

    Plasmid

    #159926

    Purpose

    Timestamp driven by a c-Fos promoter, with RNA barcode CTG, AKA 187.

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    iRFP-Timestamp A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge