We narrowed to 10,000 results for: ADA

1. pKS diaCas9_sgRNA

   Plasmid

   #74923

   Purpose

   Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

   Depositor

   Per Winge

   Article

   Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

   Inserts

   LHCF2 promoter
   diaCas9
   LHCF1 terminator
   U6 promoter
   sgRNA
   U6 3' region
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Cas9 module and sgRNA module

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. V51 pIRESpuro-GLUE (pGLUE)

   Plasmid

   #15100

   Purpose

   tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Type

   Empty backbone

   Use

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-mScarlet-EFS-Puro

   Plasmid

   #227259

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCX-ThPDE5VV

   Plasmid

   #134778

   Purpose

   cGMP FRET biosensor

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

   Insert

   ThPDE5VV
   

   Use

   Tags

   cpVenus-Venus and mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ826

   Plasmid

   #39316

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Histidine 840 to Alanine (H840A)

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. CP1041-ABEmax

   Plasmid

   #119808

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCX-SponGee

   Plasmid

   #134775

   Purpose

   mammalian expression of SponGee, a buffer of cGMP that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

   Insert

   SponGee
   

   Use

   Tags

   FLAG and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. hIRE1a wt

   Plasmid

   #20744

   Depositor

   Fumihiko Urano

   Article

   Lipson et al PLoS ONE. 2008 . 3(2):e1648.

   Insert

   IRE1 (ERN1 Human)
   

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAID-EF1a linearizing in pX330

   Plasmid

   #140610

   Purpose

   Crispr/Cas9 plasmid for linearization of pAID-EF1a

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   sgRNA for pAID-EF1a plasmid digestion
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO-FLAG-CHK1

    Plasmid

    #86875

    Purpose

    To express CHK1 with a FLAG tag at N-terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    CHEK1 (CHEK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR V5-RNF25_IRES-mCherry

    Plasmid

    #198386

    Purpose

    RNF25 expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RNF25 (RNF25 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-PPARgamma2

    Plasmid

    #78768

    Purpose

    To overexpress PPARgamma2 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

    Insert

    PPARG (Pparg Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCAM-kan-R4R3

    Plasmid

    #71275

    Purpose

    Binary destination vector for plant transformation. Kanamycin resistance.

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Type

    Empty backbone

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLENTI_RPS10_K138/139R

    Plasmid

    #127138

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K138R/K139R

    Promoter

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. ITPKA-mNeonGreen

    Plasmid

    #98883

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    ITPKA
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBa.Lamp1-GFP

    Plasmid

    #180250

    Purpose

    Mammalian expression of LAMP1

    Depositor

    Marvin Bentley

    Article

    Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. aCKAR

    Plasmid

    #124427

    Purpose

    FRET-based reporter for monitoring aPKC activity in cells

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

    Insert

    aCKAR
    

    Use

    Tags

    mCFP and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTK194

    Plasmid

    #59563

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP XbaI attB1 Kozak mKate opal attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. SaKKH-ABEmax

    Plasmid

    #119815

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. mNeonGreen-UtrCH

    Plasmid

    #98879

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    UtrCH
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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