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pHH0103_SH3RF2-3/3

Plasmid

#91281

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-3/3 (SH3RF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pKF-P14MMP2AG-T2APuroR-5h

Plasmid

#128256

Purpose

Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

rtTA-Advanced::P2A::EGFP::T2A::PuroR

Use

Synthetic Biology; Flp-in expression vector

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMiNGo11-3

Plasmid

#126058

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

mitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMiNGo11-7

Plasmid

#126057

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

mitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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USH1C_PDZ_1

Plasmid

#103936

Purpose

Protein expression and purification of P73 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

USH1C (USH1C Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC

Plasmid

#114374

Purpose

expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and TlcnC

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pfast bac-flag Fbxl3

Plasmid

#51894

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl3 (Fbxl21 Mouse)

Tags

FLAG

Promoter

polyhedrin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pTYB3-human PUM1-HD MUT3-1 (C935N)-intein-CBD

Plasmid

#73304

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

C935N

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pSTV6-3xFLAG-miniTurbo-VHL54:213

Plasmid

#248674

Purpose

To generate cells expressing Nterm tagged miniTurbo deltaVHL for ProtacID via lentivirus (no NLS)

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL(54-213) (VHL Human)

Tags

FLAG-miniTurbo

Expression

Mammalian

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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pSTV6-3xFLAG-miniTurbo-NLS-VHL54:213

Plasmid

#248673

Purpose

To generate cells expressing Nterm tagged miniTurbo deltaVHL for ProtacID via lentivirus.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL(54-213) (VHL Human)

Tags

FLAG-miniTurbo-NLS

Expression

Mammalian

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ132B-TLS-PtALSgRNA

Plasmid

#245881

Purpose

Golden gate entry vector carrying the gRNA for base editing in Poplar hybrid ALS gene along with TLS mobile RNA sequence

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

PtALSgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-2Psub-Kv-WPRE

Plasmid

#247323

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Carolan et al Nat Commun. 2025 Oct 3;16(1):8834. doi:

Insert

JEDI-2Psub-Kv

Use

AAV and Cre/Lox

Tags

Kv

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-2Psub-WPRE

Plasmid

#247224

Purpose

Double floxed genetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector under the control of the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Carolan et al Nat Commun. 2025 Oct 3;16(1):8834. doi:

Insert

JEDI-2Psub

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-LSL-Cyp26b1-PGK-H2BRFP

Plasmid

#241862

Purpose

Cre-mediated Cyp26b1 overexpression

Depositor

Elaine Fuchs

Article

Tierney et al Science. 2024 Mar 8;383(6687):eadi7342.

Insert

Cyp26b1 (Cyp26b1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_MGA

Plasmid

#240327

Purpose

Entry vector for Gateway with MGA

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

MGA (MGA Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(K55R)-Avi

Plasmid

#245186

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

K55R

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(T52S)-Avi

Plasmid

#245185

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

T52S

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-GFP

Plasmid

#243759

Purpose

AAV plasmid expressing GFP under the CAG promoter.

Depositor

Andrew Arrant

Article

Nader et al Sci Rep. 2025 Jul 19;15(1):26217. doi: 1

Insert

Enhanced Green Fluorescent Protein

Use

AAV

Promoter

CAG

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(L43F)-Avi

Plasmid

#245184

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

L43F

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(T42A)-Avi

Plasmid

#245183

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

T42A

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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