-
Plasmid#104147PurposedCas9 plasmid expressing 2A sRNA triggerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only
-
PAJ390
Plasmid#104148PurposedCas9 plasmid expressing 3A sRNA triggerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0296
Plasmid#162305PurposeLevel 0 CDS part for MoClo assembly, AATG-TTCGDepositorInsertCDS, superfolder GFP, no stop codon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV3
Plasmid#128882PurposePosition 3 module- sfGFPDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
TTN/pAS2-1
Plasmid#98127Purposetitin in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertTTN (TTN Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
MDH1-pre-let-7_PShairpin
Plasmid#26537DepositorInsertpre-let-7_PS hairpin
UseRetroviralTagsExpressionMammalianMutation5 nt mutated to make pre-let-7 stem pairing perfe…PromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAM003
Plasmid#170007PurposeEncodes PmgtC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PmgtCAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAM004
Plasmid#170008PurposeEncodes PugtL-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PugtLAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAM006
Plasmid#170010PurposeEncodes PpagC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PpagCAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAM007
Plasmid#170011PurposeEncodes PslyB-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PslyBAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[sfGFP]
Plasmid#213902PurposeMariner transposon expressing sfGFP with kan selection casetteDepositorInsertmariner transposon with kan resistance and superfolder GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas9m4
Plasmid#47316PurposeCas9 D10A+D839A+H840A+N863ADepositorInsertCas9m4
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9
Plasmid#110183PurposeExpression of the dCas9 gene in StreptomycesDepositorInsertdCas9
UseCRISPR; ITagsExpressionBacterialMutationD10A, H840APromoterermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro-humanU6-shRNA FASN
Plasmid#82327Purposeused to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)DepositorInsertshRNA to target Fatty Acid Synthase (FASN) (FASN Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro PRMT5-CRISPRi
Plasmid#164637PurposelentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2DepositorInserttarget guide sequence PRMT5 inhibition (PRMT5 Human)
UseCRISPR and Lentiviral; InterferenceTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9
Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorInsertYeast-optimized dCas9
UseTagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCJH019_SC101_pLAC_Cas12c(4)_Kan
Plasmid#183071PurposeWild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c_4
UseTagsExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC FLAG hRap1 delta BR delta CT
Plasmid#13257DepositorInsertRepressor Activator Protein 1 delta Br delta CT (TERF2IP Human)
UseRetroviralTagsFLAGExpressionMutationcontains hRap1 aa2-40 + 96-270 +331-399 and the p…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCJH021_SC101_pLAC_Cas12c2_Kan
Plasmid#183072PurposeWild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
DT042A
Plasmid#159403PurposeInducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoterDepositorInsertpr.T5-LacO-6xHis-sfGFP
UseTags6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only