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pUT1180-HQ858802

Plasmid

#27884

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

C2C2-CO-like transcription factor encoded by Os06g0298200 LOC_Os06g19444

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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EC.SurA.pTYB1.a21-428.M231R

Plasmid

#15956

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

mature SurA protein M231R mutant

Tags

intein

Expression

Bacterial

Mutation

M231R

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
M25 NacMS>Nac

Plasmid

#17175

Depositor

Randall Moon

Article

Dorsky et al Genes Dev. 2000 Jan 15;14(2):158-62.

Insert

nacre promoter driving nacre cDNA (mitfa Zebrafish)

Mutation

All three Tcf/Lef sites are mutated

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pANC98a(3xFLAG-NES-FKBP12-(G4S)4-nTEV-IRES-dTomato)

Plasmid

#248358

Purpose

Lentiviral vector that can express FKBP12 along with N terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al bioRxiv 2025.09.07.674790

Insert

3xFLAG-NES-FKBP12-(G4S)4x-nTEV

Use

Lentiviral

Promoter

tight TRE

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pSS2.1-mNeongreen

Plasmid

#241016

Purpose

Expresses mNeongreen under control of Msg promoter

Depositor

Alan Smulian

Article

Sayson et al mBio. 2025 Sep 23:e0182525. doi: 10.1128

Inserts

mNeongreen
blasticidin-S deaminase

Expression

Yeast

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pSS1-mNeongreen

Plasmid

#241014

Purpose

Expresses mNeongreen under control of Msg promoter

Depositor

Alan Smulian

Article

Sayson et al mBio. 2025 Sep 23:e0182525. doi: 10.1128

Inserts

mNeongreen
blasticidin-S deaminase

Expression

Yeast

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-TO-CasRx(NLS)-T2A-mCherry

Plasmid

#242433

Purpose

CasRx (NLS-RfxCas13d-NLS) for RNA targeting under the CMV-TO promoter (CMV with two Tet-Operators)

Depositor

Diego Di Bernardo

Article

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

CasRx

Use

CRISPR

Tags

Tag / Fusion Protein: T2A mCherry

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFN1-catP-oriT

Plasmid

#244451

Purpose

A pFN1-aad9 plasmid carrying an OriT sequence for conjugative transfer into F. nucleatum.

Depositor

Xue Liu

Insert

CatP

Expression

Bacterial

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pKH9-catP

Plasmid

#244450

Purpose

A shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a thiamphenicol resistance marker (catP gene).

Depositor

Xue Liu

Inserts

pKH09 Fn ori
CatP

Expression

Bacterial

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pFN1-Aad9

Plasmid

#244449

Purpose

A shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a spectinomycin resistance marker (aadA gene).

Depositor

Xue Liu

Inserts

pFn1 Fn ori
Aad9

Expression

Bacterial

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pFABs9

Plasmid

#241905

Purpose

Ferrulic acid biosensor - expresses sfGFP in response to substituted cinnamic acid

Depositor

Neil Dixon

Article

Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

Insert

sfGFP, ferC, ferA

Use

Synthetic Biology

Mutation

none

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p131C-B10

Plasmid

#241908

Purpose

Optimised PCA biosensor produced sfGFP in response to hydroxyl-substituted benzoic acids, including protocatechuic acid (PCA)

Depositor

Neil Dixon

Article

Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

Insert

pcaV, sfGFP

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-NiV-F-WT-Foldon

Plasmid

#200391

Purpose

Mammalian cell expression of Nipah virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

NiV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-LayV-F-WT-Foldon

Plasmid

#200392

Purpose

Mammalian cell expression of Langya virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

LayV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Mutation

Ectodomain (AA 1-483)

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJV170

Plasmid

#180077

Purpose

Backbone to clone MTase genes for expression in TXTL

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Type

Empty backbone

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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VN-dCasE-VC-GK

Plasmid

#164277

Purpose

Express VN-dCasE-VC protein in mammalian cells. This plasmid is also referred to as as VN-dEcCas6-VC-GK in the publication.

Depositor

Chunyu Han

Article

Gao et al Nucleic Acids Res. 2022 May 6;50(8):e46.

Insert

VN-dCasE-VC

Expression

Mammalian

Mutation

H20A in EcCasE

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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MZB208

Plasmid

#217844

Purpose

Bacterial expression of 10xHis-SUMO (Smt3) fusion to AP3M1 amphipathic helix (135-151) fused to mGreenLantern

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

mGreenLantern

Tags

10x His, AP3M1 (135-151), and SUMO (SMT3)

Expression

Bacterial

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_mutCKAR3

Plasmid

#238414

Purpose

AAV construct with CKAR3 mutant expression under a human synapsin promotor and WPRE3 translation enhancer.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

phosphorylation deficient mutant of CKAR3 (T to A)

Use

AAV

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR2 short

Plasmid

#236267

Purpose

Bacterial expression of bovine arrestin-2 short isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-2 (ARRB2 Bovine)

Expression

Bacterial

Mutation

short isoform

Promoter

lac

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCDS.AEBP2.I3

Plasmid

#234573

Purpose

Coding sequence for AEBP2 Isoform 3

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

AEBP2 [Isoform 3] (AEBP2 Human)

Use

Unspecified

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMB.AI3

Plasmid

#234578

Purpose

Expression of AEBP2 [Isoform 3]

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

AEBP2 [Isoform 3] (AEBP2 Human)

Expression

Insect

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGlab592

Plasmid

#236554

Purpose

240 basepair upstream sequence of KSHV ORF68 driving HaloTag expression

Depositor

Britt Glaunsinger

Article

Morgens et al Mol Syst Biol. 2025 Jan;21(1):30-58. doi

Insert

KSHV ORF68 upstream 240bp

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_iC

Plasmid

#231418

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pJAT22

Plasmid

#204311

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-GFPn

Use

CRISPR

Promoter

3XP3

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pS5

Plasmid

#231614

Purpose

L-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signals

Depositor

Hans Christopher Bernstein

Article

Chan et al mSystems. 2024 Sep 17;9(9):e0084924. doi

Insert

Pbad-TetR-sfGFP-Ptet-AraC-mKate2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pbad/Ptet

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-U6tetO_shFF4

Plasmid

#229207

Purpose

lvl 1 expresion vector

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Pol3 expresion of shFF4

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_iA

Plasmid

#231417

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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