We narrowed to 16,254 results for: She

1. lenti-dCas9-ZIM3-KRAB-BFP

   Plasmid

   #196712

   Purpose

   CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-KRAB(ZIM3)-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mek1

   Plasmid

   #40774

   Depositor

   Dustin Maly

   Insert

   MKK1 (MAP2K1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Starting Met removed

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1+ SMN1 myc HIS

   Plasmid

   #71687

   Purpose

   Mammalian expression of SMN1

   Depositor

   Gideon Dreyfuss

   Insert

   SMN1 (SMN1 Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1α-AausFP1-Z1

   Plasmid

   #135614

   Purpose

   AausFP1 - bright green fluorescent protein expression from A. australis under EF1a-HTLV promoter in minimal backbone plasmid.

   Depositor

   Jordan Green

   Insert

   AausFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-HTLV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. HaloTag-G protein gamma-2

   Plasmid

   #67018

   Purpose

   G protein gamma-2 tagged with HaloTag on amino terminus

   Depositor

   Catherine Berlot

   Insert

   HaloTag-G protein gamma-2 (GNG2 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIPZ(M)-HT-Cbx8

   Plasmid

   #82516

   Purpose

   Lentivirus vector. Expresses halotag Cbx8fusion proteins in mammalian cells.

   Depositor

   Xiaojun Ren

   Insert

   Chromobox Homolog 8 (CBX8 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pet28a_Synthetic_Human_H2AZ.1

   Plasmid

   #42629

   Depositor

   Joe Landry

   Insert

   Human H2AZ.1 (H2AZ1 Codon Optimized, Human, Synthetic)
   

   Use

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Codon Optimized

   Promoter

   T7

   Available since

   March 21, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pFastBac1_FLAG-BPTF

   Plasmid

   #102242

   Purpose

   Expression of human Flag-BPTF using baculovirus from pFastBac1

   Depositor

   Joe Landry

   Insert

   Flag-BPTF (BPTF Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedron

   Available since

   Dec. 11, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pXP118

   Plasmid

   #26825

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2010 Sep 10. ():.

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP2017 - pAAV-AiE0774m-minBG-iCre(R297T)-BGHpA

    Plasmid

    #220723

    Purpose

    AiE0774m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP Cited2

    Plasmid

    #112246

    Purpose

    expression of EGFP-Cited2 in mammalian cells

    Depositor

    Simone Di Giovanni

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-DIO-CAG-hM3Dq-P2A-dTomato

    Plasmid

    #202036

    Purpose

    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Insert

    hM3Dq
    

    Use

    AAV

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJM1-4EBP1-T37A/T46A

    Plasmid

    #136420

    Purpose

    Stable overexpression of elF4EBP1 T37A/T46A

    Depositor

    Gabriel Leprivier

    Insert

    eIF4EBP1 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T37A/T46A

    Promoter

    CMV

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. NET43 pEGFP-N2 (609)

    Plasmid

    #61991

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Insert

    NET43 (NAT10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-HA_mouse_Adar2_Tv1

    Plasmid

    #158110

    Purpose

    expresses mADAR2 shortest transcript

    Depositor

    Emanuela Felley-Bosco

    Insert

    mAdar2 (Adarb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-HIS-Sangamo

    Plasmid

    #40786

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    N Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus, Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3.3_SARS2_XBB.1.16

    Plasmid

    #201189

    Purpose

    expressing SARS-CoV-2 XBB.1.16 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (XBB.1.16) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E180V, T(K)478R on the basis of XBB.1.5

    Promoter

    CMV

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lazarus Gata 2 flag

    Plasmid

    #80137

    Purpose

    retroviral expression of Gata2-Flag

    Depositor

    Ellen Rothenberg

    Insert

    Gata2 (Gata2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-Sangamo-HIS

    Plasmid

    #40787

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    C Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus,Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #211342

    Purpose

    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection

    Depositor

    Monkol Lek

    Insert

    m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUAST_mLExA-VP16-NFATc1 (C-del)

    Plasmid

    #35023

    Depositor

    Jing-W Wang

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Tags

    VP16 and mLexA

    Expression

    Insect

    Mutation

    deletion of amino acids 590-716

    Promoter

    UAS

    Available since

    March 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid

    #180152

    Purpose

    tdTomato cre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLNCX1 HA AKT2

    Plasmid

    #60129

    Purpose

    expresses HA tagged AKT2

    Depositor

    Morris Birnbaum

    Insert

    protein kinase B (AKT2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1/HisA/Serca1

    Plasmid

    #74402

    Purpose

    expression of SERCA in mammalian cells

    Depositor

    Robert Nakamoto

    Insert

    SERCA1 (ATP2A1 rabbit)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    NcoI site destroyed (silent) T898

    Promoter

    CMV

    Available since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pAAV-DIO-CAG-hM4Di-P2A-dTomato

    Plasmid

    #202037

    Purpose

    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Insert

    hM4Di
    

    Use

    AAV

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV Erh-V5

    Plasmid

    #175123

    Purpose

    Lentiviral expression of mouse Erh-V5

    Depositor

    Larry Gerace

    Insert

    Erh (Erh Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hKv2.1-pEGFP-C1

    Plasmid

    #111538

    Purpose

    Expresses human KCNB1 in mammalian cells for imaging

    Depositor

    Federico Sesti

    Insert

    human Kv2.1 (KCNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CMV-FLEX-SaCas9-U6-sgNts

    Plasmid

    #159909

    Purpose

    Mutagenesis of Nts

    Depositor

    Larry Zweifel

    Insert

    Nts (Nts Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAP1-3

    Plasmid

    #71258

    Purpose

    luciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Insert

    3 copies of an AP-1 site from the human Stromelysin-1 gene (MMP3 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GM-CSF minimal

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7-V5-SBP-C1-HshnRNPC1

    Plasmid

    #64920

    Purpose

    Expresses V5- and SBP-tagged hnRNPC1 in mammalian cells

    Depositor

    Elisa Izaurralde

    Insert

    hnRNPC1 (HNRNPC Human)
    

    Use

    Tags

    V5 and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR_EF1a_flagLaM4-Notch-Gal4VP64

    Plasmid

    #216669

    Purpose

    Expresses anti-mCherry synNotch Gal4VP64

    Depositor

    Leonardo Morsut

    Insert

    anti-mCherry synNotch Gal4VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTRE-Tight-Rluc8

    Plasmid

    #79844

    Purpose

    Rluc8 reporter plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    Rluc8
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV Ef1a-EGFP-WPRE

    Plasmid

    #135428

    Purpose

    Can be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequence

    Depositor

    Rylan Larsen

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid

    #106964

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CBIG-Ngn2

    Plasmid

    #48708

    Purpose

    bicistronic expression of Ngn2 and EGFP

    Depositor

    Jeffrey Macklis

    Insert

    Neurog2 (Neurog2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/beta-actin

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDEST26-MSRA-C-HA

    Plasmid

    #195177

    Purpose

    Vector for transient expression of MSRA gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

    Depositor

    Stephanie Roessler

    Insert

    MSRA (MSRA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pINDUCER20-FLAG-hPAX7 (#734)

    Plasmid

    #78339

    Purpose

    Doxycycline-inducible lentiviral overexpression of human PAX7 ORF with a FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    PAX7 (PAX7 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pCDNA5 FRT TO FLAG LRRK1

    Plasmid

    #232062

    Purpose

    Expresses FLAG-LRRK1 in mammalian cells

    Depositor

    Samara Reck-Peterson

    Insert

    LRRK1 (LRRK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. OMM-SPLICSs-nFAST

    Plasmid

    #210430

    Purpose

    Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    OMM-SPLICSs-nFAST
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GFP-hChibby1

    Plasmid

    #89472

    Purpose

    Labels mother centrioles/basal bodies

    Depositor

    Ken-Ichi Takemaru

    Insert

    human Chibby1 (CBY1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge