We narrowed to 5,686 results for: org

1. plenti_DCLK1 G533N

   Plasmid

   #163627

   Purpose

   Expresses DCLK1 G533N in E. coli

   Depositor

   Kenneth Westover

   Article

   Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

   Insert

   DCLK1 (DCLK1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   G33N

   Promoter

   sv40

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO CBX1-APEX2

   Plasmid

   #129642

   Purpose

   Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   CBX1 (CBX1 Human)
   

   Tags

   1xFLAG and APEX2

   Expression

   Mammalian

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO APEX2-eIF4E1

   Plasmid

   #129644

   Purpose

   Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   EIF4E1 (EIF4E Human)
   

   Tags

   APEX2

   Expression

   Mammalian

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. XIAP (pBS0852)

   Plasmid

   #185282

   Purpose

   For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   XIAP (XIAP Human)
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2PH-PLCd-APEX2-P2A-mKate2

   Plasmid

   #71284

   Purpose

   Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   2PH-PLCd-APEX2-P2A-mKate2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-DCX-2A-mCherry

   Plasmid

   #118290

   Purpose

   Expresses human DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   doublecortin (DCX Human)
   

   Use

   AAV

   Tags

   2A peptide and mCherry

   Expression

   Mammalian

   Promoter

   CMV with beta global intron

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS mSynCAM1

   Plasmid

   #115496

   Purpose

   expression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expression

   Depositor

   Thomas Biederer

   Article

   Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

   Insert

   Cadm1 (Cadm1 Mouse)
   

   Expression

   Mammalian

   Promoter

   chicken beta-actin

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pLenti-TRE3G-BE3RA-PGK-Puro

   Plasmid

   #110846

   Purpose

   Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Mutation

   D10A

   Promoter

   TRE3G

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPEPX-P3-sgRNAluc

   Plasmid

   #85590

   Purpose

   Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for

   Depositor

   Jan-Willem Veening

   Article

   Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

   Insert

   sgRNA targeting firefly luciferase encoding gene
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   P3

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. plenti_DCLK1 G511N

    Plasmid

    #163626

    Purpose

    Expresses DCLK1 G511N in E. coli

    Depositor

    Kenneth Westover

    Article

    Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

    Insert

    DCLK1 (DCLK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    G511N

    Promoter

    sv40

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. px459 sgAtg5

    Plasmid

    #175023

    Purpose

    CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

    Depositor

    David Tumbarello

    Article

    Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

    Insert

    ATG5 (ATG5 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMTTH+mEGFP-H1.4

    Plasmid

    #157799

    Purpose

    Expression of mEGFP and human linker histone H1.4 fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-H1.4 (H1-4 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    pTAC

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

    Plasmid

    #104097

    Purpose

    Expresses TREAT-PK co-localization control in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

    Expression

    Mammalian

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Tag2-Sox9 T236A

    Plasmid

    #111455

    Purpose

    Transient expression of Flag-tagged human Sox9 with T236A mutation

    Depositor

    Sharon Pine

    Article

    Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

    Insert

    Sox9 (SOX9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T236A mutation

    Promoter

    CMV

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-Ef1a-RPS27A-UTR-PP7-WPRE

    Plasmid

    #198339

    Purpose

    PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    RPS27A-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-Puro-TDP-43-A90V

    Plasmid

    #133754

    Purpose

    Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed alanine 90 to valine

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH_TNF-SBP-mCherry

    Plasmid

    #65283

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Promoter

    CMV

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-BE3-FNLS(RA)

    Plasmid

    #112671

    Purpose

    CMV expression vector for BE3-FNLS construct (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3-FNLS
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    CMV

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCB320-sgNC.SPA

    Plasmid

    #169834

    Purpose

    Expresses a negative control sgRNA

    Depositor

    Laura Attardi

    Article

    Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

    Insert

    safe harbor sgRNA
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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