We narrowed to 18,681 results for: tec

1. EasyFusion T2A-H2B-miRFP703

   Plasmid

   #113097

   Purpose

   General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-miRFP703
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCS2+Cas9-mSA

   Plasmid

   #103882

   Purpose

   Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   CAS9-MSA
   

   Use

   CRISPR

   Tags

   MSA (monomeric streptavidin

   Expression

   Mutation

   none

   Promoter

   SP6

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV-U6+27-Tornado-RhoBAST

   Plasmid

   #196341

   Purpose

   Overexpression of circular RhoBAST

   Depositor

   Murat Sunbul

   Article

   Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

   Insert

   Tornado-RhoBAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EasyFusion T2A-H2B-mCherry

   Plasmid

   #113089

   Purpose

   General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Vimentin-7

   Plasmid

   #55156

   Purpose

   Localization: Intermediate Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pTol2-hspDRv7_scGstlt

   Plasmid

   #108870

   Purpose

   scGESTALT V7 lineage barcode for zebrafish

   Depositor

   Alex Schier

   Article

   Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

   Insert

   scGESTALT lineage tracing target V7
   

   Use

   Tol2 integration into zebrafish genome

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG1237 FIREmate-ECFP-Fis1

   Plasmid

   #205173

   Purpose

   Expresses FIREmate-ECFP-Fis1 in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   FIREmate-ECFP-Fis1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. tTA_BFP

   Plasmid

   #58854

   Purpose

   Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   tTA transcription factor fused to enhanced blue fluorescent protein
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 TaGu N-1F

   Plasmid

   #203762

   Purpose

   For expression of N-flag tagged TaGu R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19 - T7 pro - IRES - EGFP

    Plasmid

    #138586

    Purpose

    Express EGFP with an upstream T7 promoter in pUC19 backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cre Stoplight 2.4

    Plasmid

    #37402

    Purpose

    Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

    Depositor

    Thomas Hughes

    Article

    Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

    Insert

    Cre Stoplight 2.4
    

    Use

    Tags

    ZsGreen1 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYMA1_SidK_mCH

    Plasmid

    #184906

    Purpose

    Legionella pneumophila SidK (1-278) - mCherry

    Depositor

    John Rubinstein

    Article

    Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

    Insert

    SidK (1-278) (sidK Legionella pneumophila)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMIII-Neo

    Plasmid

    #153422

    Purpose

    MSCV with IRES-Neomycin resistant gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pS448∙CsR

    Plasmid

    #122096

    Purpose

    CRISPR/Cas9 counterselection in Gram-negative bacteria

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pem7 and Pm

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMCB306

    Plasmid

    #89360

    Purpose

    sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET29b-IPP1-His

    Plasmid

    #124137

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Inorganic pyrophosphatase (IPP1 Budding Yeast)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. BASU RaPID plasmid

    Plasmid

    #107250

    Purpose

    Expresses the BASU RaPID labeling protein

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    BASU
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. HBA1 full 3'UTR-T4 td downstream intron

    Plasmid

    #189974

    Purpose

    Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    HBA1 full 3'UTR-T4 td downstream intron
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a-GluRS-His

    Plasmid

    #124109

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutamyl-tRNA ligase (gltX Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-L1-PGK-PuroTK-L2

    Plasmid

    #141005

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    PuroTK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge