We narrowed to 8,294 results for: construct

1. pBabe-Kras S17N

   Plasmid

   #58899

   Purpose

   Expresses human Kras with a S17N mutation

   Depositor

   Channing Der

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S17N mutation

   Promoter

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-FlEx switch

   Plasmid

   #82406

   Purpose

   Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ033

   Plasmid

   #98404

   Purpose

   Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E-mnx1

   Plasmid

   #82402

   Purpose

   Gateway-compatible zebrafish mnx1 promoter

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   mnx1 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYZ173

   Plasmid

   #98410

   Purpose

   Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Insert

   gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-P2A-GFP no-pA

   Plasmid

   #80826

   Purpose

   P2A followed by GFP without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-P2A-GFP no-pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Gal1/10p CAS9 Gal1/10p I-SceI

   Plasmid

   #182519

   Purpose

   Galactose-Induced Expression vector for Cas9 and I-SceI.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Inserts

   I-SceI
   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. C-SWAT AID*-GFP osTIR1

   Plasmid

   #182514

   Purpose

   C-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   AID*
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-6P-SEW-YC3.6

   Plasmid

   #31379

   Depositor

   Fritjof Helmchen

   Article

   Minderer et al J Physiol. 2011 Nov 14.

   Insert

   Yellow Cameleon 3.60
   

   Use

   AAV

   Tags

   Venus and eCFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-P2A-mKate2 no-pA

    Plasmid

    #80828

    Purpose

    P2A followed by mKate2 without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p3E-P2A-mKate2 no pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMycoFos

    Plasmid

    #84577

    Purpose

    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.

    Depositor

    Nicholas Coleman

    Article

    Ly et al J Microbiol Methods. 2011 Sep;86(3):320-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pBS-ermGFP

    Plasmid

    #27172

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBYK01

    Plasmid

    #138656

    Purpose

    phiOZJ integrating Streptomyces expression vector lacking RDF

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYZ146

    Plasmid

    #98406

    Purpose

    Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.leu1 (leu1 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMD5

    Plasmid

    #138652

    Purpose

    phiBT1 integrating Streptomyces expression vector

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYZ164

    Plasmid

    #98408

    Purpose

    Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.his3 (his3 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAMP-CY1_NF1 T1 (+minintr, KDR)

    Plasmid

    #119200

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 1 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMD13

    Plasmid

    #138653

    Purpose

    phiC31 integrating Streptomyces expression vector

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available since

    June 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-EF1a-SOX2

    Plasmid

    #35388

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TALE-Sox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

20. p5E-CMVmin

    Plasmid

    #80802

    Purpose

    minimal cytomegalovirus immediate early enhancer/promoter (CMVmin) from pcDNA3

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    minimal cytomegalovirus immediate early enhancer/promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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