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Showing: 141 - 150 of 1251 results

  1. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid
    #219653
    Purpose
    General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus
    Depositor
    Thomas Oertner
    Insert
    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    Use
    AAV
    Tags
    2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)
    Expression
    Mammalian
    Mutation
    Promoter
    nEf Addgene # 149296
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    219653-plasmid-map-sequence-id-434847

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  4. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

    Plasmid
    #205703
    Purpose
    CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines
    Depositor
    Kai Johnsson
    Article
    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.
    Insert
    Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    205703-plasmid-map-sequence-id-423137

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  5. PMmEos3.2

    Plasmid
    #203755
    Purpose
    Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    Lyn n-term (LYN Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203755-plasmid-map-sequence-id-403338

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  7. pcDNA3-PM-CaNAR2

    Plasmid
    #64730
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    Lyn-CaNAR2
    Use
    Tags
    Cerulean3, N-terminal targeting sequence from Lyn…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64730-plasmid-map-sequence-id-109348

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Showing: 141 - 150 of 1251 results