Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 1,621 results for: nif

Showing: 701 - 750 of 1621 results

  2. pOPINB-HOIL-1 full-length (1–510)

    Plasmid
    #193858
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:
    Insert
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Codon optimised for expression in E. coli
    Promoter
    T7
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    193858-plasmid-map-sequence-id-379617

    Enlarge

  5. pOPINB-HOIL-1 helix-RBR (233–510)

    Plasmid
    #193859
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:
    Insert
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Residues 233–510, codon optimised for expression …
    Promoter
    T7
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    193859-plasmid-map-sequence-id-379616

    Enlarge

  10. pGP8G-ccdB

    Plasmid
    #83884
    Purpose
    Destination vector for integration at HIS3, expression from the GPD promoter
    Depositor
    Jennifer Nemhauser
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83884-plasmid-map-sequence-id-160280

    Enlarge

  11. pGP6G-ccdB

    Plasmid
    #83880
    Purpose
    Destination vector for integration at yeast URA3 locus, expression from the GPD promoter
    Depositor
    Jennifer Nemhauser
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83880-plasmid-map-sequence-id-160276

    Enlarge

  12. pGP4G-cccdB

    Plasmid
    #83876
    Purpose
    Destination vector for integration at yeast Trp locus, expression from the GPD promoter
    Depositor
    Jennifer Nemhauser
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    83876-plasmid-map-sequence-id-160272

    Enlarge

  13. pGP5G-ccdB

    Plasmid
    #83879
    Purpose
    Destination vector for integration at yeast Leu locus, expression from the GPD promoter
    Depositor
    Jennifer Nemhauser
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83879-plasmid-map-sequence-id-160275

    Enlarge

  16. pOpen-T4gene43

    Plasmid
    #165524
    Purpose
    Polymerase that replicates viral genomic DNA.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    T4 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165524-plasmid-map-sequence-id-338922

    Enlarge

  17. pOpen-Pwopol

    Plasmid
    #165562
    Purpose
    DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Pwo DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165562-plasmid-map-sequence-id-340035

    Enlarge

  18. pOpen-ECOpolA

    Plasmid
    #165532
    Purpose
    DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    E. coli DNA Polymerase I
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165532-plasmid-map-sequence-id-339121

    Enlarge

  19. pOpen-EcoRV

    Plasmid
    #165548
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    EcoRV
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165548-plasmid-map-sequence-id-339428

    Enlarge

  20. pOpen-T7gene1

    Plasmid
    #165505
    Purpose
    Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    T7 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165505-plasmid-map-sequence-id-338461

    Enlarge

  21. pOpen-KTHEAQpol

    Plasmid
    #165542
    Purpose
    Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    KlenTaq1 polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165542-plasmid-map-sequence-id-339275

    Enlarge

  22. pOpen-TthRT

    Plasmid
    #165566
    Purpose
    DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Thermus thermophilus (Tth) RT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165566-plasmid-map-sequence-id-340094

    Enlarge

  23. pOpen-SapIR

    Plasmid
    #165537
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    SapI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165537-plasmid-map-sequence-id-339127

    Enlarge

  24. pOpen-EcoRIR

    Plasmid
    #165504
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    EcoRI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165504-plasmid-map-sequence-id-338511

    Enlarge

  26. pOpen-SfiIM

    Plasmid
    #165538
    Purpose
    Site-specific DNA methyltransferase; protects DNA from cleavage by SfiI.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M.SfiI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165538-plasmid-map-sequence-id-339128

    Enlarge

  27. pOpen-XbaIR

    Plasmid
    #165567
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    XbaI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165567-plasmid-map-sequence-id-340099

    Enlarge

  28. pOpen-PstIR

    Plasmid
    #165526
    Purpose
    Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    PstI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165526-plasmid-map-sequence-id-339060

    Enlarge

  29. pOpen-HindIIIR

    Plasmid
    #165508
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    HindIII
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165508-plasmid-map-sequence-id-338587

    Enlarge

  30. pOpen-NcoIR

    Plasmid
    #165568
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    NcoI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165568-plasmid-map-sequence-id-340100

    Enlarge

  31. pOpen-EcoRVM

    Plasmid
    #165558
    Purpose
    Site-specific DNA methyltransferase; protects DNA from cleavage by EcoRV.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M.EcoRV
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165558-plasmid-map-sequence-id-339951

    Enlarge

  32. pOpen-Eco31IB

    Plasmid
    #165527
    Purpose
    Site-specific DNA methyltransferase 2; protects DNA from cleavage by Eco31I
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Eco31IB
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165527-plasmid-map-sequence-id-339063

    Enlarge

  33. pOpen-Eco31IA

    Plasmid
    #165517
    Purpose
    Site-specific DNA methyltransferase 1; protects DNA from cleavage by Eco31I
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Eco31IA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165517-plasmid-map-sequence-id-338650

    Enlarge

  34. pOpen-SapIM2

    Plasmid
    #165557
    Purpose
    Site-specific DNA methyltransferase 2; protects DNA from cleavage by SapI
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M2.SapI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165557-plasmid-map-sequence-id-339911

    Enlarge

  35. pOpen-SapIM1

    Plasmid
    #165547
    Purpose
    Site-specific DNA methyltransferase 1; protects DNA from cleavage by SapI
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M1.SapI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165547-plasmid-map-sequence-id-339425

    Enlarge

  36. pOpen-Fpg

    Plasmid
    #165541
    Purpose
    Formamidopyrimidine DNA Glycosylase; Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Fpg
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165541-plasmid-map-sequence-id-339267

    Enlarge

  37. pOpen-NotIM

    Plasmid
    #165518
    Purpose
    Site-specific DNA methyltransferase; protects DNA from cleavage by NotI
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M.NotI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165518-plasmid-map-sequence-id-338654

    Enlarge

  38. pOpen-NcoIM

    Plasmid
    #165578
    Purpose
    Site-specific DNA methyltransferase; protects DNA from cleavage by NcoI
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M.NcoI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165578-plasmid-map-sequence-id-338826

    Enlarge

  40. pOpen-XbaIM

    Plasmid
    #165577
    Purpose
    Site-specific DNA methyltransferase; protects DNA from cleavage by XbaI
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M.XbaI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165577-plasmid-map-sequence-id-341004

    Enlarge

  41. pOpen-Bst HF

    Plasmid
    #165539
    Purpose
    High fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Bst HF
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 1, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165539-plasmid-map-sequence-id-339129

    Enlarge

  42. pOpen-T4-BGT

    Plasmid
    #165530
    Purpose
    T4 Phage beta-glucosyltransferasetransfers the glucose moiety of UDP-Glc to the 5-hmC residues in double-stranded DNA.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    T4-BGT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165530-plasmid-map-sequence-id-339085

    Enlarge

  43. pOpen-9N7lig

    Plasmid
    #165514
    Purpose
    9 Degrees North DNA Ligase active at elevated temperatures (45 - 90 degrees C).
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    9 Degrees North DNA Ligase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165514-plasmid-map-sequence-id-338640

    Enlarge

  44. pOpen-Eco31I

    Plasmid
    #165506
    Purpose
    Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Eco31I
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165506-plasmid-map-sequence-id-338512

    Enlarge

  45. pOpen-9N7polA

    Plasmid
    #165500
    Purpose
    Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    9 degrees N-7 DNA polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165500-plasmid-map-sequence-id-338428

    Enlarge

  46. pOpen-THEAQlig

    Plasmid
    #165535
    Purpose
    Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Taq DNA Ligase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165535-plasmid-map-sequence-id-339125

    Enlarge

  47. pOpen-UDG

    Plasmid
    #165531
    Purpose
    Uracil DNA glycosylase catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    UDG
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165531-plasmid-map-sequence-id-339117

    Enlarge

  48. pOpen-Bstpol

    Plasmid
    #165543
    Purpose
    Full length DNA polymerase from Bacillus stearothermophilus.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Bst DNA Polymerase, Full Length
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165543-plasmid-map-sequence-id-339279

    Enlarge

  49. pOpen-TthpolA

    Plasmid
    #165533
    Purpose
    DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Tth DNA polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165533-plasmid-map-sequence-id-339122

    Enlarge

  50. pWPI-myogenin

    Plasmid
    #79073
    Purpose
    Lentiviral plasmid that constitutively expresses human myogenin
    Depositor
    Jennifer Lei
    Insert
    human myogenin (MYOG Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79073-plasmid-map-sequence-id-151242

    Enlarge

Showing: 701 - 750 of 1621 results