-
Plasmid#225663PurposeLentivirus protein expression. Matched control construct for 8522-M31-663 (Addgene # 225661) gives basal expression from mCMVp for gating/thresholdingDepositorInsertdsCherry
UseLentiviralTagsExpressionMutationPromotermCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3/gamma2SIL
Plasmid#121433PurposeGABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorUseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1/gamma2SIL
Plasmid#121432PurposeGABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorUseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
mIFP12-CD81-10
Plasmid#56248PurposeLocalization: Tetraspanin/Membrane, Excitation: 683, Emission: 704DepositorInsertCD81 (CD81 Human)
UseTagsmIFP12ExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pD649-HAsp-LEP-COMP5AP-AviTag-9xHis
Plasmid#157103PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertLEP (LEP Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-IL1R1-Fc(DAPA)-AviTag-6xHis
Plasmid#156756PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertIL1R1 (IL1R1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LRFN3-Fc(DAPA)-AviTag-6xHis
Plasmid#156903PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertLRFN3 (LRFN3 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEPT_Cdk2-T160A-siR
Plasmid#73975PurposeHDR template to generate Cdk2-T160A-siRDepositorUseAAVTagsExpressionMutationT160 mutated to Ala and siRNA target site mutatedPromoterAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIT-COMP5AP-AviTag-9xHis
Plasmid#157449PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertKIT (KIT Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma2S/deltaIL
Plasmid#119730PurposeGABAA receptor expression (chimeric rat gamma2 short subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat delta subunit) Myc-tag near N-terminusDepositorUseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEPT_Cdk2-T160E-siR
Plasmid#73976PurposeHDR template to generate Cdk2-T160E-siRDepositorUseAAVTagsExpressionMutationT160 mutated to Glu and siRNA target site mutatedPromoterAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LRFN3-COMP5AP-AviTag-9xHis
Plasmid#157467PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertLRFN3 (LRFN3 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-IL1R1-COMP5AP-AviTag-9xHis
Plasmid#157320PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertIL1R1 (IL1R1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD28-COMP5AP-AviTag-9xHis
Plasmid#157090PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertCD28 (CD28 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LEP-Fc(DAPA)-AviTag-6xHis
Plasmid#156538PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertLEP (LEP Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD28-Fc(DAPA)-AviTag-6xHis
Plasmid#156525PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCD28 (CD28 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD81-pHluorin
Plasmid#130903PurposeExpression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD81-pHluorin (CD81 aequorea victoria, Human)
UseTagspHluorinExpressionMammalianMutationpHluorin inserted between Leu-49 and Gly-50 in ex…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD81-pHuji
Plasmid#130904PurposeExpression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD81-pHuji (CD81 Synthetic, Human)
UseTagspHujiExpressionMammalianMutationpHuji inserted between Leu-49 and Gly-50 in extra…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIT-Fc(DAPA)-AviTag-6xHis
Plasmid#156885PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertKIT (KIT Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF1A-Fc(DAPA)-AviTag-6xHis
Plasmid#156598PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertTNFRSF1A (TNFRSF1A Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only