Addgene

We narrowed to 3,980 results for: Bre;

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pDest17_Gcn4 E2K2

Plasmid

#231868

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E2K2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 E2K2

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S104K, S117E, S136E, S144K

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Aro3+

Plasmid

#231884

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro3+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 Aro3+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Aro2+

Plasmid

#231883

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro2+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 Aro2+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 F108A+

Plasmid

#231875

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F108A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 F108A+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108A, T121L, A141P

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-del(IV)

Plasmid

#111321

Purpose

snu114-del(IV)

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

deletion of domain IVa

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNa_opt.IRES.eGFP

Plasmid

#22489

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

codon optimised wild type Calcineurin A (PPP3CA Human)

Use

Retroviral

Tags

Expression

Mutation

Codon Optimised wtCNa

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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p3113 GST-p53

Plasmid

#10852

Depositor

Peter Howley

Article

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

p53 (TP53 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-RanQ69L

Plasmid

#30309

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

RanQ69L (RAN N/A)

Use

Tags

FLAG-Cherry

Expression

Mammalian

Mutation

Glutamine 69 is changed to Leucine

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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mNG2(1-10)_tdTomato

Plasmid

#184169

Purpose

Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(1-10)
tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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SFG.CNb30_opt.IRES.eGFP

Plasmid

#22493

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)

Use

Retroviral

Tags

Expression

Mutation

L124T; K125-LA-Ins

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pFBP_promoter_acceptor

Plasmid

#189920

Purpose

Binary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformation

Depositor

Adam Steinbrenner

Article

Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-CalfluxCTN

Plasmid

#190068

Purpose

Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

Depositor

Carl Johnson

Article

Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

Insert

CalfluxCTN

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 WT

Plasmid

#195213

Purpose

Mammalian expression vector for GFP tagged NHERF1.

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV Sport2 mPer1

Plasmid

#16203

Depositor

Cheng Lee

Article

Albrecht et al Cell. 1997 Dec 26. 91(7):1055-64.

Insert

Per1 (Per1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only
pJB06

Plasmid

#190480

Purpose

Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Cas9
XylR

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Tags

Expression

Mutation

Promoter

xylR-driven promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJB07

Plasmid

#190481

Purpose

Targeting plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Upstream & Downstream pyrE deletion region
gRNA

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hBcan

Plasmid

#18966

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Jul 7. ():.

Insert

Brevican (BCAN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
sgDonor_lentiGuide

Plasmid

#184168

Purpose

LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Insert

sgDonor

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-Cherry-N1-PRKAG1

Plasmid

#30308

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase γ1 (PRKAA1 Human)

Use

Tags

FLAG and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
pMB4

Plasmid

#122246

Purpose

Lentivirus delivery for stable expression of As crRNA, has AsDR; Cloning vector for expression of AsCpf1 crRNA. It contains BsmBI site for cloning.

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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