We narrowed to 2,418 results for: EFS

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pKG3779_pGEEC586_pShip-CMV(promoter)-EFS(UTR)-mRuby2-bGH

Plasmid

#239748

Purpose

Plasmid expressing mRuby2 from the CMV canonical promoter with the EFS 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1301-AAV-EFSNC-dCjCas9-NIPP1(143-224)

Plasmid

#223146

Purpose

Expression of truncated NIPP1 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

NIPP1 (PPP1R8 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 143-224

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)

Plasmid

#223145

Purpose

Expression of truncated MBD2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD2 (MBD2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 176-231

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

Plasmid

#223143

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 111-173

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

Plasmid

#223144

Purpose

Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD1 (MBD1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 529-592

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1306-AAV-EFSNC-dSaCas9-HP1aNH(115-177)

Plasmid

#223151

Purpose

Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)

Plasmid

#223141

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1309-AAV-EFSNC-dSaCas9-MBD1(529-592)

Plasmid

#223154

Purpose

Expression of truncated MBD1 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD1 (MBD1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 529-592

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1308-AAV-EFSNC-dSaCas9-HP1bNH(111-173)

Plasmid

#223153

Purpose

Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 111-173

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1313-AAV-EFSNC-dSaCas9-MECP2(204-310)

Plasmid

#223158

Purpose

Expression of truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1311-AAV-EFSNC-dSaCas9-NIPP1(143-224)

Plasmid

#223156

Purpose

Expression of truncated NIPP1 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

NIPP1 (PPP1R8 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 143-224

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

Plasmid

#223155

Purpose

Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD2 (MBD2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 176-231

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)

Plasmid

#214882

Purpose

Lentiviral vector encoding RfxCas13d targeting PROX guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crPROX-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211688

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

Plasmid

#126759

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

HeFSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

Plasmid

#113696

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

Plasmid

#113695

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

Plasmid

#138147

Purpose

Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

Plasmid

#138148

Purpose

Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NES

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

Plasmid

#223164

Purpose

2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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