We narrowed to 513 results for: PA-GFP

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pAAV-syn-AICD-IRES-hrGFP

Plasmid

#107548

Purpose

high transduction efficiency AAV-mediated synapsin promoter-dependent expression of AICD (last 50 amino acids of APP) and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD (APP Human)

Use

AAV

Mutation

None

Promoter

human synapsin

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Gfi1-IRES-GFP

Plasmid

#91891

Purpose

Retroviral expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

MMLV gag

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NES-IRES-hrGFP

Plasmid

#107544

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NES (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-p53DN-T2A-copGFP

Plasmid

#109229

Purpose

For Tol2 transposon-mediated stable expression of dominant negative p53 and copGFP

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

dominant negative p53 (Trp53 Mouse)

Tags

copGFP

Expression

Mammalian

Mutation

dominant negative p53

Promoter

CAGGS

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMX-HA-UbvG08-IRES-GFP

Plasmid

#75030

Purpose

Retroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53)

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

Ubiquitin

Use

Retroviral

Tags

HA and IRES-eGFP

Mutation

UbvG08 with I44A mutation and no terminal Glycines

Available Since

June 30, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pMX-HA-UbvG08_MUT-IRES-GFP

Plasmid

#75031

Purpose

Retroviral vector with ubiquitin variant with reverted mutations L69P and V70L (i53-DM mutant)

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

Ubiquitin

Use

Retroviral

Tags

HA and IRES-eGFP

Mutation

UbvG08 with I44A, P69L, and L70V mutations and no…

Available Since

June 30, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-mouse Ezh2 Y637S-IRES-GFP

Plasmid

#91887

Purpose

Retroviral expression of mouse Ezh2 with Y637S

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Y637S

Promoter

MMLV gag

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*GFP

Plasmid

#162806

Purpose

STELLA plasmid for amber suppression of N-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

EF1_Ub-(N-TAG)GFP

Use

Piggybac

Expression

Mammalian

Mutation

GFP (Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-human Erbb2-IRES-GFP

Plasmid

#91888

Purpose

Retroviral expression of human Erbb2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Erbb2 (ERBB2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

MMLV gag

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2 delta SETdomain-IRES-GFP

Plasmid

#91885

Purpose

Retroviral expression of mouse Ezh2 with a SET domain deletion

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

SET domain deleted

Promoter

MMLV gag

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP

Plasmid

#184811

Purpose

Used to generate the Tg(QUAS:GFP)c578 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-pA

Use

Zebrafish tol2 transgenesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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372 pTol2-fli1ep:EGFPDest

Plasmid

#73491

Purpose

Tol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:EGFP-attR1-attR2

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

Plasmid

#91879

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

Plasmid

#91880

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

Plasmid

#91884

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP

Plasmid

#91877

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

Plasmid

#91883

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3

Plasmid

#67276

Purpose

RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA

Depositor

Jannik Elverløv-Jakobsen

Article

Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

Inserts

GFP (mWasabi)
hygro

Tags

F3 and Frt

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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HLH Clock Pas 1-401 VenN L74E

Plasmid

#47355

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L74E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only