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Plasmid#103242PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-143-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-143-3p target (MIR143 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATF4 11: 5’ATF4.uORF2.GFP
Plasmid#21858DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationupstream ORF2 (uORF) of ATF4 fused to EGFPPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-186-3p
Plasmid#103288PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-186-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-186-3p target (MIR186 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTR
Plasmid#67932PurposeTranslational reporter - Lox 5'UTRDepositorInsertsfGFP-NLS-P2A-NLS-mCherry (LOX Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
K560CLM T324C
Plasmid#24460DepositorInsertKinesin (KIF5B Human)
UseTagsHisExpressionBacterialMutationC7S/C65A/C168A/C174S/C294A/C330S T324C/C421APromoterAvailable sinceAug. 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-155-3p
Plasmid#103264PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-155-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-155-3p target (MIR155 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLM RP HTR
Plasmid#24430DepositorInsertKinesin (KIF5B Human)
UseTags6XHisExpressionBacterialMutationC7S/C65A/C168A/C174S/C294A/C330S/C421APromoterAvailable sinceJune 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 C-terminal half
Plasmid#74599Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523DepositorInserthuman dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationamino acids 389-523 of LIC1PromoterT7Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-138-5p
Plasmid#103233PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-138-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-138-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-148a-3p
Plasmid#103250PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-148a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-148a-3p target (MIR148A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBD21_033
Plasmid#109150PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of SiriusDepositorInsertsmRFP
Sirius
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_036
Plasmid#109153PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2DepositorInsertsmRFP
mKO2
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_034
Plasmid#109151PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFPDepositorInsertsmRFP
TagBFP
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 8: pCEFL.PSMB2.GST
Plasmid#21798DepositorInsertPSMB2 (Psmb2 Mouse)
UseTagsGSTExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
ATF4 18: RLuc.5’ATF4.GFP
Plasmid#21864DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
ATF4 14: 5’ATF4.uORF1&2AUA.GFP
Plasmid#21861DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationAUG codons of uORFs1&2 were converted to AUAPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-449a
Plasmid#103528PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-449a target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-449a target (MIR449A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK101
Plasmid#59561PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4DepositorInsertSGP(14) mKate-G8-L7Ae-PEST 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT p85-GFP
Plasmid#126660PurposeInducible expression of p85-GFP in S2 cellsDepositorInsertPI3K SH2 containing subunit
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only