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pcDNA5FRTTO-codoptUL33-CStHA

Plasmid

#135106

Purpose

Codon-optimized HSV-1 UL33 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL33 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUS2-CStHA

Plasmid

#135107

Purpose

Codon-optimized HSV-1 US2 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 US2 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2235

Plasmid

#111888

Purpose

High copy number plasmid containing Ple320 (POU4F1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Ple320 (POU4F1 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2236

Plasmid

#111889

Purpose

High copy number plasmid containing Ple321 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple321 (TUBB3 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Ple321 (TUBB3 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2023

Plasmid

#49130

Purpose

AAV plasmid with hs1218 promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-hs1218-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hs1218

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2024

Plasmid

#49131

Purpose

AAV plasmid with hs671 promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-hs671-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hs671

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2025

Plasmid

#49132

Purpose

AAV plasmid with NOV (Ple303) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple303-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

NOV

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2050

Plasmid

#49135

Purpose

AAV plasmid with CAGGS promoter driving expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-emGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

Simian CMV IE94

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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NA20 LN16

Plasmid

#101870

Purpose

Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

Depositor

Paul Clapham

Article

Peters et al J Virol. 2004 Jul;78(13):6915-26.

Insert

Env (env )

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only
TetO-FUW-NfiA

Plasmid

#64901

Purpose

Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

Nfia (Nfia Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NfiB

Plasmid

#64900

Purpose

Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

NfiB (Nfib Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_CyOFP1_KCNQ2 var4

Plasmid

#173155

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Use

Tags

IRES2-CyOFP1

Expression

Mammalian

Mutation

Promoter

Simian CMV IE94

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(+) NRP1 b1 (273-427)

Plasmid

#162734

Purpose

Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Article

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1 domain residues 273-427

Use

Tags

Expression

Bacterial

Mutation

Codon optimised

Promoter

Available since

April 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a(+) NRP1 b1b2 (273-586)

Plasmid

#162733

Purpose

Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Article

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1b2 domains (residues 273-586)

Use

Tags

6His

Expression

Bacterial

Mutation

Codon optimised

Promoter

Available since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2284

Plasmid

#158419

Purpose

AAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple349 (PDE6H Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple349

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2214

Plasmid

#158417

Purpose

AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple265 (PCP2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple265

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2271

Plasmid

#158418

Purpose

AAV plasmid with Ple341 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple341 (PCP2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple341

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2159

Plasmid

#70120

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-iCre-WPRE

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

May 12, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2260

Plasmid

#158423

Purpose

AAV plasmid with Ple331 (PAX6 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple331 (PAX6 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple331

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge