We narrowed to 159,157 results for: addgene

1. pcDNA3.1-Clover-mRuby2

   Plasmid

   #49089

   Purpose

   Clover-mRuby2 tandem fusion for FRET calibration

   Depositor

   Kurt Beam

   Insert

   Clover-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-GFAP-mNeonGreen

   Plasmid

   #105713

   Purpose

   AAV packaging vector to express mNeonGreen in astrocytes.

   Depositor

   Pantelis Tsoulfas

   Insert

   mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Minimal GFAP promoter

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1_V5_APEX_FLAG_Nt

   Plasmid

   #194068

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5-APEX-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-fDIO-EYFP-WPRE

   Plasmid

   #154870

   Purpose

   Human synapsin-1 promoter; Flp-dependent EYFP

   Depositor

   Ulrik Gether

   Insert

   EYFP
   

   Use

   AAV and Mouse Targeting; Flp-frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human synapsin promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SIRT6

   Plasmid

   #28271

   Depositor

   Cheryl Arrowsmith

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pCW57.1_V5_APEX_FLAG_Ct

   Plasmid

   #194069

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG-APEX-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX551-miniCMV-AsCpf1

   Plasmid

   #107034

   Purpose

   AAV plasmid expressing AsCpf1 under control of miniCMV promoter

   Depositor

   Alex Hewitt

   Insert

   AsCpf1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV Rnf121-V5

   Plasmid

   #175130

   Purpose

   Lentiviral expression of mouse Rnf121-V5

   Depositor

   Larry Gerace

   Insert

   Rnf121 (Rnf121 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTre-Tight-luciferase-GFP (467)

   Plasmid

   #65491

   Purpose

   negative control for expression assays

   Depositor

   Rory Johnson, Roderic Guigo

   Insert

   EGFP
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJM104: pTrc RFP on pCM66T

    Plasmid

    #74745

    Purpose

    pCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)

    Depositor

    Mary Lidstrom

    Insert

    RFP (BioBrick part number BBa_E1010)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX551-CMV-CjCas9

    Plasmid

    #107035

    Purpose

    AAV plasmid expressing CjCas9 under control of CMV promoter

    Depositor

    Alex Hewitt

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV Nup62-V5

    Plasmid

    #175116

    Purpose

    Lentiviral expression of mouse Nup62-V5

    Depositor

    Larry Gerace

    Insert

    Nup62 (Nup62 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRR2

    Plasmid

    #166097

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX551-miniCMV-CjCas9

    Plasmid

    #107033

    Purpose

    AAV plasmid expressing CjCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX551-miniCMV-SaCas9

    Plasmid

    #107032

    Purpose

    AAV plasmid expressing SaCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. NADK

    Plasmid

    #28268

    Depositor

    Cheryl Arrowsmith

    Insert

    NADK:HPC0AC-F12:C216828 (NADK Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains residues 68-426.

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pLV Tor1a-V5

    Plasmid

    #175135

    Purpose

    Lentiviral expression of Tor1a-Myc

    Depositor

    Larry Gerace

    Insert

    Tor1a (Tor1a Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pITI1

    Plasmid

    #166094

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. cTRE-GFP-miR30_shPten.1523

    Plasmid

    #135667

    Purpose

    Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    shPten
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PP-CDPK1 (3Q5I)

    Plasmid

    #28280

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-CDPK1 (PB000158.03.0 Plasmodium berghei)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits