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Plasmid#201965PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationE198A, R199A,E200A, D201APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA
Plasmid#201967PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationR232A, K233A, R234A, K235A, R236APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA
Plasmid#201968PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationW255A,N262E,K266EPromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
myc-Miro2 (lacking TM)
Plasmid#127614PurposeHuman Miro2 with the transmembrane deletedDepositorInsertMiro2 (RHOT2 Human)
UseTagsmycExpressionMammalianMutationTransmembrane deletionPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
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EGFP-hPMCA2w/b
Plasmid#47586DepositorInsertPMCA2w/b (ATP2B2 Human)
UseTagsEGFP-HA-HisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(36-235)
Plasmid#170533PurposeExpresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsHis tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-Hyg-Dest-mCherry-PAR-6B
Plasmid#194908PurposeTo generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.DepositorInsertPAR-6B (Pard6b Mouse)
UseMouse TargetingTagsmCherryExpressionMammalianMutation39 C to G, silent mutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2GPI
Plasmid#203759PurposeEncodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.DepositorInsertCD58 c-term (CD58 Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry2-sgMUC4
Plasmid#198399PurposeExpresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporterDepositorInsertMUC4 sgRNA (MUC4 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPMCA2x/b
Plasmid#47585DepositorInsertpMCA2x/b (ATP2B2 Human)
UseTagsEGFP-HA-HisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPMCA2z/b
Plasmid#47584DepositorInsertpMCA2z/b (ATP2B2 Human)
UseTagsEGFP-HA-HisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG eGFP-Oct4
Plasmid#203789PurposeCompetitive assay of Oct4 and Oct6 binding to DNA motifs.DepositorInsertPou5f1 (Pou5f1 Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.1
Plasmid#88994PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.1 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.1
Plasmid#88997PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.1 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- WTN23C11orf83-GFP
Plasmid#65845PurposeMammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorInsertTransmembrane (23 AA) of UQCC3 (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationWT TransmembranePromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51 GFP-PNRC1
Plasmid#123295PurposeMammalian expression of PNRC1 N-GFPDepositorInsertPNRC1 (PNRC1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG eGFP-Oct6
Plasmid#203790PurposeCompetitive assay of Oct4 and Oct6 binding to DNA motifs.DepositorInsertPou3f1 (Pou3f1 Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only