Addgene

We narrowed to 1,468 results for: pBluescript

Showing: 281 - 300 of 1468 results

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371 pTol2-fli1ep:MTDest

Plasmid

#73490

Purpose

Tol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:6xMyc-attR1-attR2

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPEE7

Plasmid

#128357

Purpose

Recycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

ACT1

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-GGAC-TTCT

Plasmid

#60945

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-CGGA-GTGC

Plasmid

#60950

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pMLS257

Plasmid

#73716

Purpose

SapTrap destination vector for building repair template only vectors

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SapI repair template insertion site

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pBlue-hFX-HA(NcoI/EcoRI)

Plasmid

#15405

Depositor

Michael Ristow

Insert

human frataxin cDNA (FXN Human)

Use

Tags

AU1 and HA

Expression

Bacterial

Mutation

insert can be released by digestion with REs NcoI…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pRVV647

Plasmid

#87630

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBS CHX10 3'UTR (CC#56)

Plasmid

#15150

Depositor

Connie Cepko

Article

Chen et al Mech Dev. 2000 Feb . 90(2):293-7.

Insert

CHX10 3'UTR (VSX2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pBSWt1-Dest

Plasmid

#59764

Purpose

PCR template for amplifying the attL1-em7-kanr-attL2-pA cassette

Depositor

Peter Koopman

Article

Zhao et al Dev Dyn. 2014 Jun 12. doi: 10.1002/dvdy.

Insert

attL1-em7-kanR-attL2-pA

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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3063 pBS Hox11 genomic

Plasmid

#8748

Depositor

Stanley Korsmeyer

Article

Roberts et al Nature 1994 Apr 21;368(6473):747-9.

Insert

Hox11 (genomic fragment) (Tlx1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
PL08009A2A04

Plasmid

#44667

Depositor

Phillip Newmark

Article

Chong et al BMC Dev Biol. 2011 Nov 10;11:69.

Insert

Smed-pka

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pFloxin

Plasmid

#24553

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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fabp10a:ER-CreER; cryaa:mCerulean

Plasmid

#230077

Purpose

Expression of ERCreER in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Hovhannisyan et al bioRxiv 2025.05.23.655316

Insert

ERT2-Cre-ERT2

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

fabp10a

Available since

May 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pgRNA_AT

Plasmid

#196243

Purpose

Backbone plasmid for cloning sgRNA

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRVV653

Plasmid

#89474

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRVV598

Plasmid

#87629

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_XcmI-LacZ

Plasmid

#49296

Purpose

Golden Gate entry vector at position 1, no 5' ATG, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_Linker

Plasmid

#49281

Purpose

Golden Gate entry vector at position 1, small linker insert, no 5' ATG

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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PL08003A1B11

Plasmid

#44681

Depositor

Phillip Newmark

Article

Chong et al BMC Dev Biol. 2011 Nov 10;11:69.

Insert

Smed-plastin

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
PL05007B1D03

Plasmid

#44680

Depositor

Phillip Newmark

Article

Chong et al BMC Dev Biol. 2011 Nov 10;11:69.

Insert

Smed-tsp-1

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only