We narrowed to 5,876 results for: nls

1. DNMT3L-dCas9

   Plasmid

   #126587

   Purpose

   DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

   Insert

   DNMT3L (DNMT3L Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-DNMT3L-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pST1374-scFv-APOBEC-UGI-GB1

   Plasmid

   #113029

   Purpose

   Expresses scFv-rAPOBEC1-UGI in mammalian cells

   Depositor

   Xingxu Huang

   Article

   Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

   Insert

   scFv-rAPOBEC1-UGI-GB1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. BII-BCA-Prom-ASCL2

   Plasmid

   #133394

   Purpose

   Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

   Depositor

   Blair Madison

   Article

   Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

   Insert

   destabilized tdTomato-NLS and destabilized NLS-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free) drives expressi…

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHEN-CoV2-nanobody-F1

   Plasmid

   #194592

   Purpose

   Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   F1 (S )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-HF-BE3

   Plasmid

   #87439

   Purpose

   Expresses HF-BE3 with C-terminal NLS in mammalian cells

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   HF-BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mammalian codon-optimized Cas9-HF

   Promoter

   CMV

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-NRCH-APOBEC-nCas9-Ung

   Plasmid

   #198552

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-APOBEC-nCas9-Ung
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221 MLK1

   Plasmid

   #60540

   Purpose

   Donor vector for human MLK1

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   MLK1 (MAP3K9 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pMKO shRNA Bim

   Plasmid

   #17235

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bim shRNA (BCL2L11 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Expression

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-FGFR4

    Plasmid

    #60531

    Purpose

    Entry vector for human FGFR4

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    FGFR4 (FGFR4 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    c2134a P712T

    Promoter

    NA

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pHEN-CoV2-nanobody-E2

    Plasmid

    #194590

    Purpose

    Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    E2 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. hnRNPA2_LC_WT

    Plasmid

    #98657

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST1374-GCN4-D10A

    Plasmid

    #113022

    Purpose

    Expresses GCN4-D10A in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    GCN4-D10A (GCN4 Budding Yeast, S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. BE3-P2A-EGFP (pJUL977)

    Plasmid

    #123612

    Purpose

    CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMH-SFB-USP7

    Plasmid

    #99393

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    USP7 (USP7 Human)
    

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. hnRNPA2_LC_nocharge

    Plasmid

    #139118

    Purpose

    express hnRNPA2 LC with no charged residues

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all charged residues are changed to S/Q except fo…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS1859

    Plasmid

    #205972

    Purpose

    FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Csy3/Cas7 from JAGBLH010000220, Cas8-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS1860

    Plasmid

    #205973

    Purpose

    FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9-DNMT3L

    Plasmid

    #126588

    Purpose

    DNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

    Insert

    DNMT3L (DNMT3L Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-dCas9-DNMT3L-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-IFT54

    Plasmid

    #218726

    Purpose

    Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

    Insert

    IFT54 (TRAF3IP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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