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NFKB3-CK-IRES-GFP hygro

Plasmid

#246914

Purpose

NFKB-dependent expression of a bicistronic cDNA encoding a suicide gene (modified deoxycytidine kinase) and EGFP

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

Mutant Deoxycytidine Kinase (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

Plasmid

#228362

Purpose

mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Rfxcas13d grna expression …

Expression

Mammalian

Promoter

mU6 and hU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMB1: All in one SpCas9 expression and sgRNA cloning backbone

Plasmid

#228363

Purpose

mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-Flag-BRIP1

Plasmid

#222619

Purpose

Mammalian expression vector of flag-tagged BRIP1

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

BRIP1 (BRIP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAVA-LGF2-Gal11p

Plasmid

#127481

Purpose

Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Insert

LGF2-Gal11p (GAL11 Budding Yeast)

Expression

Bacterial

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBT346.6-H3.3-K4M-bGHpolyA

Plasmid

#128743

Purpose

Inducible expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

K4M

Promoter

CAG

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_N_ARPC1B

Plasmid

#111681

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

ARPC1B (ARPC1B Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-1

Plasmid

#87334

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMIR-31-Reporter

Plasmid

#71871

Purpose

Mammalian expression vector for the analysis of miR-31 activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-31

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-Cep123-C2 domain

Plasmid

#73329

Purpose

Encodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFP

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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mTFP1-VASP-5

Plasmid

#55513

Purpose

Localization: Actin/Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

VASP

Tags

mTFP1

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-ZO1-C-14

Plasmid

#55518

Purpose

Localization: Tight Junctions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

ZO1 (TJP1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-H1-10

Plasmid

#55487

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H1 (H1f0 Mouse)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
Cs2+Etv5

Plasmid

#49095

Purpose

expression of Etv5

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv5 (etv5a Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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sgRNA-Dual_filler(hU6_H1/7SK hybrid)-EF1as_Thy1.1_P2A_Neo

Plasmid

#239609

Purpose

Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and minimal H1/7SK hybrid promoters) using BsmbI. Selectable with a G418 resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

filler-trcr-H1/7SKhybrid-filler

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-pTau nanobody (A2) with sortase tag

Plasmid

#233218

Purpose

Mammalian epression of anti-pTau nanobody (A2) with a sortase tag for direct dye conjugation.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv [Preprint]. 2025 Feb 20:2023.10.

Insert

Anti-pTau nanobody (A2)

Tags

Sortase tag, His tag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Galt1-HT7-mGold

Plasmid

#230027

Purpose

Expressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.

Depositor

Pablo Rivera-Fuentes

Insert

Galt1-HaloTag7-mGold (B4GALT1 Synthetic, Human)

Tags

HaloTag,mGold

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHelper-CBE-sgRNA

Plasmid

#221137

Purpose

Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

sgRNA-scaffold with Cas9 handle

Use

CRISPR

Expression

Bacterial

Promoter

J23119(SpeI)

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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