We narrowed to 32,062 results for: eng

1. pYPQ266E

   Plasmid

   #161521

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   zSpRYCas9(D10A)-PmCDA1-UGI
   

   Use

   CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

   Plasmid

   #178518

   Purpose

   For making Rabies virus SAD B19 strain L cell line with hyPBase

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   B19L-IRES-mCherry
   

   Expression

   Mammalian

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PITX3-eGFP-PGK-Puro

   Plasmid

   #31942

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Insert

   PITX3 (PITX3 Human)
   

   Use

   Targeting donor

   Tags

   eGFP

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSUPER-retro-puro-shOrai1

   Plasmid

   #89933

   Purpose

   Knockdown human Orai1 expression

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   Orai1 shRNA (ORAI1 Human)
   

   Use

   Retroviral

   Available Since

   May 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYPQ265E6

   Plasmid

   #213469

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cda-32aa linker-z…

   Tags

   No

   Expression

   Plant

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDisplay-GACh1.0

   Plasmid

   #106072

   Purpose

   express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor (GACh) 1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSUPER-retro-puro-shSTIM1

   Plasmid

   #89816

   Purpose

   Knockdown human STIM1 expression

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   STIM1 shRNA (STIM1 Human)
   

   Use

   Retroviral

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

   Plasmid

   #99229

   Purpose

   Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos2
   

   Tags

   Lifeact

   Expression

   Mammalian

   Mutation

   A69T for primed conversion applications

   Promoter

   CMV

   Available Since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSUPER-retro-puro-Smad4

   Plasmid

   #89829

   Purpose

   Knockdown human Smad4 expression in human mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

   Insert

   Smad4 shRNA (SMAD4 Mouse, Human)
   

   Use

   Retroviral

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSET-pr-mKikGR1 (mKikGR1-V69T)

    Plasmid

    #99227

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mKikGR1
    

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T (Eos notatio…

    Promoter

    T7

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-mGSDMD1-251

    Plasmid

    #111565

    Purpose

    Express ELANE-cleaved mouse GSDMD in mammalian cells

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

12. B4GALT1-Dox

    Plasmid

    #124633

    Purpose

    Doxycycline-inducible B4GALT1 expression in mammalian cells

    Depositor

    Ron Weiss

    Article

    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

    Inserts

    Beta-(1,4)-Galactosyltransferase 1
    Tetracycline repressor A3 mutant
    TagFP635
    

    Expression

    Mammalian

    Promoter

    TRE-tight and hEF1a

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Tags

    Cmyc

    Mutation

    N343Q

    Promoter

    GAL1

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBRPyCAG-fmKlf4-DsRed-Ip

    Plasmid

    #26274

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ262m

    Plasmid

    #161522

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-IFITM3-F8-TAG-C72A

    Plasmid

    #122477

    Purpose

    expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Phenylalanine 8 to amber codon, cysteine …

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmiR-96 cluster promoter

    Plasmid

    #62517

    Purpose

    microRNA 183-96-182 cluster promoter luciferase plasmid

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2014 Mar;42(5):2988-9

    Insert

    promoter of microRNA-183-96-182 cluster
    

    Tags

    RenSP (optimized renilla luciferase gene)

    Expression

    Mammalian

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. PatoM-LOG241

    Plasmid

    #72847

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoM
    

    Expression

    Bacterial

    Promoter

    PatoM

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. IFITM3-N21del-V93-TAG

    Plasmid

    #122480

    Purpose

    expresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    IFITM3 (IFITM3 Human)
    

    Expression

    Mammalian

    Mutation

    Changed Valine 93 to amber codon and deleted amin…

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CNC3G (CASANOVA-C3 with glycine linkers)

    Plasmid

    #137192

    Purpose

    Expresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory protein

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

    Insert

    CNC3G (CASANOVA-C3 with glycine linkers)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge