We narrowed to 5,563 results for: org

1. pLV-EF1a-Itprip-V5

   Plasmid

   #120241

   Purpose

   Expresses V5-tagged Itprip in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Itprip (ITPRIP Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

   Plasmid

   #115142

   Purpose

   CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Mb3Cas12a
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ265G2

   Plasmid

   #164723

   Purpose

   Gateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   A3A(Y130F)-ziSpyMac(D10A)-UGI)
   

   Use

   CRISPR; Gateway compatible a3a/y130f-ispymaccas9-…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Tag2-Sox9 T236A/T240A

   Plasmid

   #111458

   Purpose

   Transient expression of Flag-tagged human Sox9 with T236A/T240A mutation

   Depositor

   Sharon Pine

   Article

   Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

   Insert

   Sox9 (SOX9 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T236A and T240A

   Promoter

   CMV

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-BE3RA-P2A-Puro

   Plasmid

   #110839

   Purpose

   Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30a-SARS-CoV2-ORF3b (S23Q)

   Plasmid

   #165088

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Stop codon to glutamine at 23rd amino acid

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDEST40-CES2A-ΔC

   Plasmid

   #200554

   Purpose

   Mammalian expression of secreted mouse CES2A

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
   

   Use

   Tags

   6xHis, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET32a-Spike-403-505

   Plasmid

   #165095

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLenti-BE3RA-PGK-Puro

   Plasmid

   #110843

   Purpose

   Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AfrLEA3m_mCh-2xFKBP (pBS1137)

    Plasmid

    #185321

    Purpose

    For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    AfrLEA3m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST_APOE_HUMAN_E4_47-300 (pBS0847)

    Plasmid

    #185279

    Purpose

    For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    APOE_HUMAN_E4_47-300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. miniSOG-Alpha-Actinin-19

    Plasmid

    #57762

    Purpose

    Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

    Plasmid

    #127790

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-VTKS5-TVA-eGFP-N2cG

    Plasmid

    #175440

    Purpose

    Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

    Depositor

    Gordon Fishell

    Article

    Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

    Insert

    TVA-P2A-eGFP-P2A-N2cG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-DYRK2-2A-GFP

    Plasmid

    #118284

    Purpose

    Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAHS2parrc-mCh-2xFKBP (pBS1109)

    Plasmid

    #185304

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2parrc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFJ889

    Plasmid

    #84826

    Purpose

    Minimos transposon with Psmu-1:smu-1:GFP:smu-1 UTR and cbr-unc-119 selection

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    smu-1 (smu-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Psmu-1

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. mKate2-P2A-APEX2-2xML1N(7Q)

    Plasmid

    #67666

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1Nmut*2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-DYRK4-2A-GFP

    Plasmid

    #118288

    Purpose

    Expresses mouse DYRK4 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (Dyrk4 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. P1_Firre_1.49_pDONR221

    Plasmid

    #195204

    Purpose

    Plasmid with pDONR221 backbone containing Firre 1.49 clone. Kanamycin resistance. Gateway cloning system.

    Depositor

    John Rinn

    Article

    Hacisuleyman et al Nat Struct Mol Biol. 2014 Feb;21(2):198-

    Insert

    mFirre 1.49 Clone
    

    Use

    Gateway cloning intermediate

    Tags

    Expression

    Mutation

    Only exons

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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