We narrowed to 13,546 results for: CAR

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pAC-ZETAipi

Plasmid

#53284

Purpose

Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

pds

Use

Low copy number bacterial cloning vector

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P12

Plasmid

#120023

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTE1062

Plasmid

#172356

Purpose

Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al Methods Mol Biol. 2018;1673:117-128. doi

Inserts

scbR (SCO6265 )
kasOp

Use

Synthetic Biology; Integrative into streptomyces …

Expression

Bacterial

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-CBP

Plasmid

#169211

Purpose

Expression of eGFP-CBP protein for chitin visualization

Depositor

James S Fraser

Article

Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

Insert

Chitinase A1 Chitin Binding Domain

Tags

6xHis, Mxe Gyr Intein, and eGFP

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBlueRIP.TfrOVA

Plasmid

#69596

Purpose

contains membrane bound form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-mOVA mice)

Depositor

Francis R. Carbone

Article

Kurts et al J Exp Med. 1996 Sep 1;184(3):923-30.

Insert

OVA (OVAL Chicken)

Tags

Transmembrane domain of Transferrin Receptor (TfR…

Expression

Mammalian

Promoter

rat insulin promoter (RIP)

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-mPer2ER-pBABEpuro

Plasmid

#61430

Purpose

Express mPER2-ER* fusion protein

Depositor

Aziz Sancar

Article

Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

Insert

mPER2 (Per2 Mouse)

Use

Retroviral

Tags

Flag and mouse estrogen receptor ligand binding d…

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pYC34

Plasmid

#63915

Purpose

Recipient overexpressing vector to construct mCherry tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

mCherry

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pQE80l A-CC-A

Plasmid

#196478

Purpose

Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

Depositor

Fei Sun

Article

Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

Insert

A-CC-A

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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GST-Cav FL (1-178)

Plasmid

#22445

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1(1-178)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z11

Plasmid

#120054

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPI452

Plasmid

#113203

Purpose

Inducible expression vector (dox-on) N-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

mScarlet

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Flag-Timeless

Plasmid

#22892

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Timeless (TIMELESS Human)

Tags

Flag and His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pPI458

Plasmid

#113212

Purpose

safe haven knock-in plasmid for the act5 locus, C-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, c…

Tags

mScarlet

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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YPRCΔ15α1

Plasmid

#101712

Purpose

GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-HCA_sensor

Plasmid

#127182

Purpose

Eukaryotic expression of the fusion protein used for sulfa-drugs sensing

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA

Tags

HisTag and StrepTag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pYC121

Plasmid

#63917

Purpose

Recipient overexpressing vector to construct MYC tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

MYC

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pES.HSV2.3a

Plasmid

#69593

Purpose

TCR a chain construct for generation of gBT-I mice

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)

Expression

Mammalian

Promoter

H-2Kb

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only
pET/LEGH

Plasmid

#108226

Purpose

Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

Depositor

Didier Picard

Article

Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

Insert

LBT-EGFP-His6

Expression

Bacterial

Promoter

T7, lacO

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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YPRCΔ15Ω1

Plasmid

#101710

Purpose

GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFBCPDL6

Plasmid

#67285

Purpose

CPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expression

Depositor

Aziz Sancar

Article

Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.

Insert

CPD photolyase (phr Fly)

Use

Bacterial vector, insert not expressed

Tags

Flag

Mutation

V275E*

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only