We narrowed to 3,133 results for: eed
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Plasmid#215880PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-99a-WT (MIR99A Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-376a1(5p)-I-type
Plasmid#215882PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of 376a1(5p)-I-type (MIR376A1 Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-22(3p)-I-type
Plasmid#215886PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-22(3p)-I-type (MIR22 Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-376a2(3p)-I-type
Plasmid#215887PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-I-type (MIR376A2 Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-99a-I-type
Plasmid#215888PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-99a-I-type (MIR99A Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)
Plasmid#225436PurposeBeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.DepositorInsertBeYDV geminino 1.0
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
opt.a_pSTC0-zeo
Plasmid#202347PurposeDesign opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertopt.a
Available SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Mut
Plasmid#149396PurposeP. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHFDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationIHFprox site is mutated at key positions needed f…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_BnHD (pBS0280)
Plasmid#185155PurposeFor the mammalian expression of the rapeseed protein BnHD. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertBnHD
ExpressionMammalianAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-miR-SC
Plasmid#65055PurposeExpression of miR148a with scrambled seed in mammalian cellsDepositorAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 6xUTR
Plasmid#40761PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert6 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 2xUTR
Plasmid#40757PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert2 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 4xUTR
Plasmid#40759PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert4 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 5xUTR
Plasmid#40760PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert5 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 1xUTR
Plasmid#40756PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsertCXCR4 small RNA target site
UseLuciferaseTagsluciferaseMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m640-1+2
Plasmid#42106DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in two seed-comp…PromoterSV40Available SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m640-2
Plasmid#42105DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m215-1+2
Plasmid#42103DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in two seed-comp…PromoterSV40Available SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m640-1
Plasmid#42104DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m215-1
Plasmid#42101DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only