We narrowed to 10,632 results for: t7

1. pJK304

   Plasmid

   #72225

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala368>Asp mutant (H6AaAlr-A368D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Expression

   Bacterial

   Mutation

   changes alanine-368 to aspartate

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK303

   Plasmid

   #72224

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala367>Asp mutant (H6AaAlr-A367D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Expression

   Bacterial

   Mutation

   changes alanine-367 to aspartate

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK301

   Plasmid

   #71555

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with C-terminal His tag (AaAlrH6)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK500

   Plasmid

   #72245

   Purpose

   Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and endogenous

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK625

   Plasmid

   #72541

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK618

   Plasmid

   #72535

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His6 tag; glutaminase subunit; large subunit, C-terminal His6 tag (H6AaPurS, AaPurQ, AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK600

   Plasmid

   #71605

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, histidine…

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTmaM4

   Plasmid

   #67714

   Purpose

   Expression vector hexahistidine tagged variant of the Thermotoga maritima M-domain protein

   Depositor

   Robert Batey, Jeffrey Kieft

   Article

   Kieft et al RNA. 2004 Jun;10(6):988-95.

   Insert

   Thermotoga marítima Ffh-M domain
   

   Tags

   hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7 RNA polymerase

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5C7M (UBC)

   Plasmid

   #69967

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ubv.it.02:YTC006-E08:C225422 (UBC Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. 4Z02

    Plasmid

    #69956

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    BRD1:JMC023-A05:C211705 (BRD1 Human)
    

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pT7-Venus-cdc25C5

    Plasmid

    #40969

    Depositor

    Olivier Gavet

    Insert

    cdc25C5 (CDC25C Human)
    

    Use

    In vitro transcription

    Tags

    Venus

    Promoter

    T7

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

12. 4MI0

    Plasmid

    #60097

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EZH2:JMC033-A03:C216172 (EZH2 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. 3UR4

    Plasmid

    #60121

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5WDR5:APC041-G05:C36415 (WDR5 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. 3HX4

    Plasmid

    #60137

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TgCDPK4:MAC03F-A01:C208088
    

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. APC041-G05

    Plasmid

    #58759

    Purpose

    bacterial expression of PDB 4O45

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4O45 (WDR5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available Since

    Aug. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. FN 221

    Plasmid

    #50498

    Depositor

    Kenneth Yamada

    Insert

    fibronectin (FN1 Human)
    

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    repeats 6-10, R1524K, D1526E, C1232A

    Promoter

    T7

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pJORSF19

    Plasmid

    #45614

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    PhoRadA precursor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. ECD8-pXT7

    Plasmid

    #32992

    Depositor

    Sergei Sokol

    Article

    Itoh et al Genes Dev. 1999 Sep 1;13(17):2328-36.

    Insert

    Ecd8 (fzd8.L Frog)
    

    Use

    Xenopus rna expression

    Mutation

    Contains the first 230 aa of Xfz8

    Promoter

    T7

    Available Since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

19. Xfz8-pXT7

    Plasmid

    #32991

    Depositor

    Sergei Sokol

    Article

    Itoh et al Mech Dev. 1998 Jun;74(1-2):145-57.

    Insert

    Xfz8 (fzd8.L Frog)
    

    Use

    Xenopus rna expression

    Promoter

    T7

    Available Since

    Jan. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pRSF-KpDcr1deltaC(GiVL-1)

    Plasmid

    #32032

    Depositor

    David Bartel

    Article

    Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

    Insert

    DCR1(15-355)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    H123-N143 replaced by P642-Y645 of GiDicer

    Promoter

    T7

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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