We narrowed to 5,789 results for: actin

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pCXLE-SOX2-p2a-KLF4

Plasmid

#193292

Purpose

episomal hSK (expressing human SOX2 + KLF4)

Depositor

Hans Schöler

Publication

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-p2a-KLF4 (SOX2, KLF4 Human)

Expression

Mammalian

Mutation

F2A of original pCXLE-SK vector was replaced with…

Promoter

CAG

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-SA-neo-CAGGS-PE2-2A-GFP

Plasmid

#180014

Purpose

Targeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus

Depositor

Dirk Hockemeyer

Publication

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

PE2-2A-GFP

Use

CRISPR; Human genome engineering

Tags

GFP

Promoter

CAG

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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UPB-colon (UPB4)

Plasmid

#180786

Purpose

Unique Projection Barcode (UPB4)-Colon for Projection-seq

Depositor

Rui Chang

Publication

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

hM3Dq ( partial )

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-PGE

Plasmid

#184617

Purpose

For expression of a fluorescent sensor for prostaglandin E2 factor in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Publication

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-PGE

Expression

Mammalian

Promoter

CAG promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-Stargazin-mEGFP-iLID

Plasmid

#178529

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Publication

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Expression

Mammalian

Promoter

CAG

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

Plasmid

#176458

Purpose

Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

Depositor

Hui Yang

Publication

Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

Insert

humanized S. pyogenes Cas9-XTEN-Ec73RT fusion

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-human-CHMP3

Plasmid

#154180

Purpose

expresses human CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Publication

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (CHMP3 Human)

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Promoter

CAG

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-SADB19_oG-IRES-Puro

Plasmid

#172367

Purpose

Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

Depositor

Yoav Ben-Simon, Peter Jonas

Publication

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

oG + Puro

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pTalpha1-Dre

Plasmid

#133925

Purpose

Neuron-specific expression of Dre

Depositor

Frank Bradke, Sebastián Dupraz

Publication

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

HA-Dre

Tags

HA

Expression

Mammalian

Promoter

CAG promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-TWIN-STREP-FLAG-FLCN

Plasmid

#164981

Purpose

FLCN expressing vector for mammalian cells

Depositor

Roberto Zoncu

Publication

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FLCN (FLCN Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS D614 soluble Spike

Plasmid

#164652

Purpose

Expresses SARS-CoV-2 soluble Spike protein

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 soluble spike (S SARS-CoV-2)

Tags

HIS

Expression

Mammalian

Promoter

CAG

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCAG-mCherry2-G

Plasmid

#164162

Purpose

Expresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminal

Depositor

Isei Tanida

Publication

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Expression

Mammalian

Promoter

CAG

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pT2ADW-4146NES(ROCK)

Plasmid

#162485

Purpose

For generating transgenic mice expressing ROCK biosensor based on FRET.

Depositor

Michiyuki Matsuda

Publication

Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

Insert

ROCK biosensor based on FRET.

Tags

YPet and ECFP

Expression

Mammalian

Promoter

CAG

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pROSA26-FlpERT2

Plasmid

#149438

Purpose

ROSA26 mouse targeting vector with tamoxifen-inducible Flp recombinase

Depositor

Primo Schaer

Publication

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Flp-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Double UP mNeon to mScarlet FRT

Plasmid

#141111

Purpose

Natively produces mNeonGreen, in the presence of Flp or Flpe will be recombined to produce mScarlet.

Depositor

Erik Dent

Publication

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG/Luci

Plasmid

#139981

Purpose

Expresses luciferase under the promoter of CAG. Transgene flanked by AAV2 ITR

Depositor

William Pu

Publication

Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

Insert

Luciferase

Use

AAV

Promoter

CAG

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

Plasmid

#133789

Purpose

CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Publication

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized CjeCas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

n/a

Promoter

CAG

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-SponGee-Kras

Plasmid

#134777

Purpose

mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling

Depositor

Xavier Nicol

Publication

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

SponGee-Kras

Tags

FLAG and mRFP

Expression

Mammalian

Promoter

CAG

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hA3A-BE3 (pRZ215)

Plasmid

#131314

Purpose

CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Publication

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP

Plasmid

#122954

Purpose

Lentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expression

Depositor

Pierre Vanderhaeghen

Publication

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Insert

NOTCH2NLB (NOTCH2NLB Human)

Use

Lentiviral

Tags

6xhis tag

Promoter

CAG promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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