We narrowed to 17,102 results for: emb

1. pMSCVpuro-Gcn5(D608A)

   Plasmid

   #63707

   Purpose

   retroviral expression of enzyme-dead mouse Gcn5 in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   Gcn5 (Kat2a Mouse)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   D608A mutation

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pRS423-mitoT

   Plasmid

   #174525

   Purpose

   Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

   Depositor

   Oleh Khalimonchuk

   Article

   Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

   Insert

   Yeast mitochondrial intermembrane tether
   

   Use

   Synthetic Biology

   Tags

   eGFP

   Expression

   Yeast

   Promoter

   MET25

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTH728-CEN-RLuc/maxCFLuc

   Plasmid

   #38212

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-USPL1-C236A-pcDNA3.1

   Plasmid

   #85764

   Purpose

   mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   catalytic inactive mutant C236A

   Promoter

   CMV

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-USPL1-C236S-pcDNA3.1

   Plasmid

   #85763

   Purpose

   mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   catalytic inactive mutant C236S

   Promoter

   CMV

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/CIP2A(1-905) R522D V5 His

   Plasmid

   #119289

   Purpose

   CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

   Depositor

   Jukka Westermarck

   Article

   Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

   Insert

   KIAA1524 (CIP2A Human)
   

   Tags

   V5 His

   Expression

   Mammalian

   Mutation

   R522D

   Promoter

   T7

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKK-NoTag

   Plasmid

   #105767

   Purpose

   Control vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Expression

   Mammalian

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH726-CEN-RLuc/minCFLuc

   Plasmid

   #38210

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-nAChr-Alpha6

   Plasmid

   #48812

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. FB009

    Plasmid

    #119707

    Purpose

    Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC::nptII::Ttub
    

    Use

    Synthetic Biology

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK-FLAG-TEV

    Plasmid

    #105768

    Purpose

    Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-hPMCA2xb

    Plasmid

    #47751

    Purpose

    mammalian expression of mCherry tagged hPMCA2, xb splice variant

    Depositor

    Emanuel Strehler

    Article

    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    xb splice variant

    Promoter

    CMV

    Available Since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pJET-41-DExCon-antiGFPnanobody-mCherry-R11b

    Plasmid

    #179901

    Purpose

    Donor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry module

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    antiGFPnanobody-mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3GS

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJET-07-mNeonGreen-R11a

    Plasmid

    #179890

    Purpose

    Donor with homologous arms for Rab11a to knock in mNeonGreen

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-TEV-mEGFP

    Plasmid

    #105794

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mEGFP

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-CRY2-P14P5K

    Plasmid

    #79569

    Purpose

    expression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

    Depositor

    Olof Idevall-Hagren

    Article

    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

    Insert

    Pip5k1c (Pip5k1c Mouse)
    

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    R445E and R446E; truncation of amino acids 636-66…

    Promoter

    CMV

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-N3/hArf5(WT)-EGFP

    Plasmid

    #79534

    Purpose

    Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf5 (ARF5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid

    #59357

    Purpose

    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acids 30-33 to confer s…

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. ORAI1-YFP T184M

    Plasmid

    #114181

    Purpose

    ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).

    Depositor

    Nicolas Demaurex

    Article

    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

    Insert

    ORAI1-YFP T184M (ORAI1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    T184M

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMP108 Nanog-Venus-2a-mCherry

    Plasmid

    #159743

    Purpose

    Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.

    Depositor

    Sergi Regot

    Article

    Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    Venus-2a-mCherry

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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