We narrowed to 8,158 results for: Dos;

1. pBa.MARK2-Halo

   Plasmid

   #224414

   Purpose

   Mammalian expression of MARK2

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   MARK2 (MARK2 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-gRNA

   Plasmid

   #53062

   Purpose

   Transcription of guide RNA (gRNA) in wheat cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   wheat U6 promoter - gRNA
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   wheat U6

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pP2A-mCherry-N1

   Plasmid

   #84329

   Purpose

   Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   P2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Fuw-dCas9-Dnmt3a_IM

   Plasmid

   #84478

   Purpose

   Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a_IM
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. p35S_GFP-GUS

   Plasmid

   #167122

   Purpose

   Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

   Depositor

   Olivier Voinnet, Arturo Marí-Ordóñez

   Article

   Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

   Insert

   GFP-GUS
   

   Use

   Tags

   mGFP5, GUS

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKRaION2-N1

   Plasmid

   #177802

   Purpose

   Mammalian expression of green fluorescent potassium indicator KRaION2

   Depositor

   Edward Boyden

   Article

   Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

   Insert

   KRaION2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-SIRT6-H133Y

   Plasmid

   #102327

   Purpose

   Human SIRT6 H133Y for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   changed histidine 133 to tyrosine

   Promoter

   CMV

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET16b-PCNA

   Plasmid

   #134898

   Purpose

   Bacterial expression and purification of human PCNA

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   PCNA (PCNA Human)
   

   Use

   Tags

   untagged

   Expression

   Bacterial

   Mutation

   fully synthetic, codon optimized for bacterial ex…

   Promoter

   T7

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXJ40 GADD34 (514-674aa)

   Plasmid

   #75480

   Purpose

   mammalian expression of GADD34 514-674 aa

   Depositor

   Shirish Shenolikar

   Article

   Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

   Insert

   GADD34 (PPP1R15A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   expresses aa 514-674

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pXJ40 GADD34 (1-513aa)

    Plasmid

    #75479

    Purpose

    mammalian expression of GADD34 1-513 aa

    Depositor

    Shirish Shenolikar

    Article

    Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

    Insert

    GADD34 (PPP1R15A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    expresses aa 1-513

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. dCas9-10xSunTag(22aa)

    Plasmid

    #215745

    Purpose

    Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    dCas9-10xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-Ki67-mCherry

    Plasmid

    #183748

    Purpose

    Expresses EGFP-Ki67-mCherry

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Use

    Tags

    Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRV2-GG

    Plasmid

    #105349

    Purpose

    virus-induced gene silencing

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    TRV-RNA2(5')
    

    Use

    In planta gene silencing; viral

    Tags

    Expression

    Mutation

    BsaI restriction sites removed

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-FAS-GCaMP6f-WPRE

    Plasmid

    #216277

    Purpose

    AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    human synapsin 1 (hSyn)

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWUR703

    Plasmid

    #53082

    Purpose

    inducible TtAgoDM (D478A,D546A) expression

    Depositor

    John van der Oost

    Article

    Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

    Insert

    TtAgo
    

    Use

    Tags

    Strep-II tag

    Expression

    Bacterial

    Mutation

    D478A D546A

    Promoter

    T7

    Available since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_CamKIIa_GFP

    Plasmid

    #96941

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Oleksandr Shcheglovitov, Ricardo Dolmetsch

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    Green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. mNeonGreen-Paxillin

    Plasmid

    #176106

    Purpose

    Focal Adhesion marker

    Depositor

    Dorus Gadella

    Article

    Arts et al Front Immunol. 2021 May 18;12:667213. do

    Insert

    Paxillin (PXN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TrkB mEGFP

    Plasmid

    #83952

    Purpose

    FRET donor for dynamically measuring TrkB activity in neurons

    Depositor

    Ryohei Yasuda

    Article

    Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

    Insert

    Tropomyosin related kinase B (Ntrk2 Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_VASP_WH1

    Plasmid

    #109790

    Purpose

    Protein expression and purification of VASP_WH1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    VASP_WH1 (VASP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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