We narrowed to 8,027 results for: Dos;

1. Fuw-dCas9-Tet1CD_IM

   Plasmid

   #84479

   Purpose

   Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Tet1CD_IM
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS_VP35_EBOV

   Plasmid

   #103050

   Purpose

   Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   VP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGWB120

   Plasmid

   #68574

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Modified EAR motif plant-specific repression doma…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAS9id

   Plasmid

   #141251

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS_3E5E_luciferase

   Plasmid

   #103055

   Purpose

   Encodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   Ebolavirus minigenome
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS_VP30_EBOV

   Plasmid

   #103051

   Purpose

   Encodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   VP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_PLK1_Pkinase

   Plasmid

   #109933

   Purpose

   Protein expression and purification of PLK1_Pkinase

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PLK1_Pkinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MP9956:SFG.GD3synthase-2A-GD2synthase

   Plasmid

   #75013

   Purpose

   Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.

   Depositor

   Martin Pule

   Article

   Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do

   Inserts

   murine GD3 synthase
   murine GD2 synthase
   

   Use

   Retroviral; Momlv retroviral vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-EN479

   Plasmid

   #86234

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nickase and sgRNA against mouse rosa26 locus
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mVenus-IQ6 WT

    Plasmid

    #198201

    Purpose

    FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    MYO5A IQ domain (Myo5a Mouse)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWUR703

    Plasmid

    #53082

    Purpose

    inducible TtAgoDM (D478A,D546A) expression

    Depositor

    John van der Oost

    Article

    Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

    Insert

    TtAgo
    

    Use

    Tags

    Strep-II tag

    Expression

    Bacterial

    Mutation

    D478A D546A

    Promoter

    T7

    Available since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPEPY-PF6-lacI

    Plasmid

    #85589

    Purpose

    Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PF6

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-BRCA1

    Plasmid

    #119007

    Purpose

    Mammalian expression of human BRCA1 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

    Plasmid

    #173873

    Purpose

    Mammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCPH2A.X-GS3

    Plasmid

    #204745

    Purpose

    Expression of Cas9 and human H2AX

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p35S_GFP-GUS

    Plasmid

    #167122

    Purpose

    Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

    Depositor

    Olivier Voinnet, Arturo Marí-Ordóñez

    Article

    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

    Insert

    GFP-GUS
    

    Use

    Tags

    mGFP5, GUS

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJWV102-PL-dCas9

    Plasmid

    #85588

    Purpose

    Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    dCas9sp
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLsp

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. LZF97_hSyn-spine-jRGECO1a

    Plasmid

    #119198

    Purpose

    Targets jRGECO1a to spines in living neurons.

    Depositor

    Don Arnold

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L) (Utrn Synthetic, Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin 1

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9-10xSunTag(22aa)

    Plasmid

    #215745

    Purpose

    Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    dCas9-10xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-L3-SpCas9-HF1-2A-EGFP-L2

    Plasmid

    #141048

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    SpCas9-HF1-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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