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PET-B-HeFSpCas9-NLS-6xHis

Plasmid

#207392

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CMV Flag PAI-RBP

Plasmid

#45452

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP (SERBP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pDisplay-GRAB_VIP1.0

Plasmid

#208682

Purpose

Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-hMC4R

Plasmid

#179315

Purpose

Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

Depositor

Christian Gruber

Article

Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

Insert

melanocortin 4 receptor (MC4R Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_R118G_Barcode

Plasmid

#170970

Purpose

Overexpression of RUNX1 R118G mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R118G

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_G100V_Barcode

Plasmid

#170955

Purpose

Overexpression of RUNX1 G100V mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G100V

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (mPCSK9)

Plasmid

#170122

Purpose

AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (Pcsk9 Mouse)

Use

AAV

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-NUDT5

Plasmid

#219955

Purpose

For protein purification from Ecoli

Depositor

Karla Feijs

Article

Weixler et al Commun Biol. 2025 Mar 18;8(1):453. doi:

Insert

NUDT5 (NUDT5 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_VIP1.0

Plasmid

#208681

Purpose

Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_-DIO-GRAB_NPY1.0

Plasmid

#208677

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0

Use

AAV

Promoter

EF1_

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

Plasmid

#176588

Purpose

Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associate IscB (Delaware Bay) locus

Expression

Bacterial

Mutation

Replaced spacer in CRISPR array with Fn spacer

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLL7 lenti-dcas9-3xflag-blast

Plasmid

#112133

Purpose

lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

dCas9(D10A, N863A)-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A mutants in Cas9

Promoter

EF1a

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-LAP3

Plasmid

#217660

Purpose

For gateway cloning of LAP3

Depositor

Karla Feijs

Article

Weixler et al Commun Biol. 2025 Mar 18;8(1):453. doi:

Insert

LAP3 (LAP3 Human)

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_EF1aUBA3_IRES_Puro

Plasmid

#235660

Purpose

human UBA3 cloned into pLV-EF1a-IRES-Puro vector (Addgene: 85132)

Depositor

Lorenz Studer

Article

Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

Insert

UBA3 (UBA3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1 (SB251)

Plasmid

#227600

Purpose

Inducible expression of Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-CD300C-V5

Plasmid

#124089

Purpose

CD300C-V5 tagged expression vector

Depositor

J. Chad Brenner

Article

Mann et al Oral Oncol. 2023 Nov;146:106562. doi: 10

Insert

CD300C (CD300C Human)

Use

Lentiviral

Tags

V5

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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MSP2440

Plasmid

#72250

Purpose

Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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VP16-ER beta (long)

Plasmid

#11352

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER beta long (ESR2 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP TEM4 FL

Plasmid

#58893

Purpose

Expresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.

Depositor

Channing Der

Article

Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

Insert

TEM4 (ARHGEF17 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L722F

Promoter

CMV

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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B-SpCas9-HF1

Plasmid

#126762

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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