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pLenti PGK mKate2-OGT K842M (Puro)

Plasmid

#154291

Purpose

Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x FLAG and mKate2

Expression

Mammalian

Mutation

K842M mutation (Catalytic Inactive)

Promoter

PGK

Available Since

Dec. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti PGK FKBP12(F36V)-OGT (Neo)

Plasmid

#154294

Purpose

Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x HA and FKBP12(F36V)

Expression

Mammalian

Mutation

Promoter

PGK

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Ef1a-hMGT-PGK-puro

Plasmid

#172394

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-FRA1

Plasmid

#188665

Purpose

Expresses murine FRA1 (Fosl1)

Depositor

Günter Schneider

Article

Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

Insert

Fosl1 (Fosl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hPGK

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-MKK6(DD)-Puro

Plasmid

#86094

Purpose

Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

Depositor

Cindy Miranti

Article

Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

Insert

MKK6 (MAP2K6 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Serine 207 and Threonine 211 both changed to Aspa…

Promoter

CMV/TetO

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DHFR.dn-cHSF1

Plasmid

#134729

Purpose

Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-CMVtight-PLXNB2 iOE

Plasmid

#176849

Purpose

lentiviral vector for doxycycline-inducible PLXNB2 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

full length PLXNB2

Promoter

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-hPLXNB2_PGK_Neo

Plasmid

#176848

Purpose

lentiviral vector for constitutive PLXNB2 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

full length PLXNB2

Promoter

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Hygro DHFR.dn-cHSF1

Plasmid

#134731

Purpose

Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Available Since

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP1A-WT-puro

Plasmid

#156166

Purpose

Lentiviral vector for expression of RAP1A-WT, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP1A-E63-puro

Plasmid

#156167

Purpose

Lentiviral vector for expression of RAP1A-E63, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Glutamine 63 to Glutamic acid

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DNFGFR1-HA

Plasmid

#130887

Purpose

Expresses dominant negative mutant mouse FGFR1

Depositor

Gian-Paolo Dotto

Article

Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

Insert

Fgfr1 (Fgfr1 Mouse)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Dominant Negative receptor lacking cytosolic cata…

Promoter

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Lamin A wildtype

Plasmid

#118709

Purpose

Lentiviral TET-ON inducible GFP-Lamin A wildtype

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Lamin A human wildtype (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBoBi-hLAMP2-C-GC6s

Plasmid

#154151

Purpose

To detect the calcium ion releases close to the lysosomal surface

Depositor

Sheng-cai Lin

Article

Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

Insert

LAMP2 and GCamp6s (LAMP2 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

CMV

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMVtight-HNF1A-FLAG-Hygro

Plasmid

#183232

Purpose

Lentiviral vector; Tet-on advanced system driving the expression of tagged HNF1A

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox A (HNF1A Human)

Use

Lentiviral

Tags

AviTag and FLAG tag

Expression

Mutation

Promoter

tight TRE

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-WT-neo

Plasmid

#156177

Purpose

Lentiviral vector for expression of RAP2A-WT, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Progerin C661S

Plasmid

#118711

Purpose

Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Progerin C661S (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Mutation

C661S farnesylation mutant

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti PGK mKate2-OGT D554N (Puro)

Plasmid

#154292

Purpose

Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x FLAG and mKate2

Expression

Mammalian

Mutation

D554N mutation (Ser/Thr glycosylation-incompetent)

Promoter

PGK

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti PGK mKate2-OGT 5N5A (Puro)

Plasmid

#154293

Purpose

Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x FLAG and mKate2

Expression

Mammalian

Mutation

N322A, N356A, N390A, N424A, N458A mutations; HCF-…

Promoter

PGK

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP1A-WT-neo

Plasmid

#156173

Purpose

Lentiviral vector for expression of RAP1A-WT, with neomycin section

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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