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DDX3X - E186A in pENTR

Plasmid

#216577

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway entry plasmid

Mutation

E186A

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X - I179T in pENTR

Plasmid

#216576

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway entry plasmid

Mutation

I179T

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X - R145C in PENTR

Plasmid

#216575

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway entry plasmid

Mutation

R145C

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X - G123E in pENTR

Plasmid

#216574

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway entry plasmid

Mutation

G123E

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X - I107V in pENTR

Plasmid

#216573

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway entry plasmid

Mutation

I107V

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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FB405

Plasmid

#203626

Purpose

Promoter of the glucoamylase gene from A. niger, maltose/starch-inducible.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PglaA

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET30a-RAMA

Plasmid

#112226

Purpose

Expresses RAMA domain from murine MPND

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

MPND (Mpnd Mouse)

Tags

Flag and HIs S-tag

Expression

Bacterial

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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CTL2

Plasmid

#92212

Purpose

Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

Depositor

Douglas Rees

Article

Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

Insert

CTL2 of PIEZO1

Tags

6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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36INS-pTet_GB

Plasmid

#78681

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [G:36INS-pTet:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pTet Promoter

Use

Synthetic Biology

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-pTet_FB

Plasmid

#78680

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [F:36INS-pTet:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pTet Promoter

Use

Synthetic Biology

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-pTet_EB

Plasmid

#78679

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [E:36INS-pTet:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pTet Promoter

Use

Synthetic Biology

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-pTet_AB

Plasmid

#78678

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [A:36INS-pTet:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pTet Promoter

Use

Synthetic Biology

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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36INS1-pBAD_GB

Plasmid

#78673

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [G:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS1-pBAD_FB

Plasmid

#78672

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [F:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS1-pBAD_EB

Plasmid

#78671

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [E:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Lf-MetQ

Plasmid

#78305

Purpose

Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

Depositor

Douglas Rees

Article

Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

Insert

Methionine Binding Protein (metQ )

Tags

6x Histidine Tag

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-A

Plasmid

#64979

Purpose

Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E108A

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-K

Plasmid

#64978

Purpose

Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pBS BMPR (DM#137)

Plasmid

#15059

Depositor

Douglas Melton

Article

Graff et al Cell. 1994 Oct 7. 79(1):169-79.

Insert

BMP receptor (bmpr1a Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pSP64T3 BMP2 pro dnmVg1 (DM#182)

Plasmid

#15074

Depositor

Douglas Melton

Article

Thomsen et al Cell. 1993 Aug 13. 74(3):433-41.

Insert

BMP2-Vg1 dom neg

Use

Sp6 expression for use in xenopus

Mutation

dominant negative version of BMP2-Vg1 chimera. Th…

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only