Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,945 results for: MLS

Showing: 3061 - 3080 of 4945 results

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pRK5_msfGFP-HMGB1-Shuffled 1

Plasmid

#237649

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 1 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Shuffled 2

Plasmid

#237650

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 2 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Shuffled 10

Plasmid

#237658

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 10

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 10 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-LacI-Cyclin B1 ND

Plasmid

#234706

Purpose

allows tethering of non degradable (ND) cyclin B1 protein to lacO repeats in specific chromatin loci

Depositor

Lienhard Schmitz

Article

Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

Insert

Cyclin B1 (CCNB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 E484Q (CAA)

Plasmid

#212423

Purpose

Encodes SARS-CoV-2 spike E484Q for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484Q (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484Q

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 deltaE156-F157+R158G (GGA)

Plasmid

#212421

Purpose

Encodes SARS-CoV-2 spike E156-F157deletion,R158G for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E156-F157deletion,R158G (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E156-F157deletion,R158G

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L981F (TTC)

Plasmid

#212486

Purpose

Encodes SARS-CoV-2 spike L981F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L981F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L981F

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T572I (ATC)

Plasmid

#212404

Purpose

Encodes SARS-CoV-2 spike T572I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T572I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T572I

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (Yellowhammer)

Plasmid

#212402

Purpose

Encodes SARS-CoV-2 spike L5F,D614G,Q677H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L5F,D614G,Q677H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L5F,D614G,Q677H

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T29I (ATA)

Plasmid

#212403

Purpose

Encodes SARS-CoV-2 spike T29I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T29I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T29I

Promoter

CAG

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 V1104L (TTA)

Plasmid

#223274

Purpose

Encodes SARS-CoV-2 spike V1104L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike V1104L (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

V1104L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Y453F (TTC)

Plasmid

#212369

Purpose

Encodes SARS-CoV-2 spike Y453F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Y453F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Y453F

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 F456L (CTT)

Plasmid

#212542

Purpose

Encodes SARS-CoV-2 Spike F456L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F456L (CTT) (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F456L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 FL.1.5.1

Plasmid

#212539

Purpose

Encodes SARS-CoV-2 variant FL1.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike FL.1.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-SAM-Puro

Plasmid

#198713

Purpose

For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

Depositor

Tyler Jacks

Article

Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

Inserts

U6-MS2-gRNAscaffold
EF1a-MPH-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a and U6

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q183H (CAT)

Plasmid

#223279

Purpose

Encodes SARS-CoV-2 spike Q183H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q183H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Q183H

Promoter

CAG

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q498R (CGA)

Plasmid

#212475

Purpose

Encodes SARS-CoV-2 spike Q498R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q498R (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Q498R

Promoter

CAG

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 A67V (GTT)

Plasmid

#212395

Purpose

Encodes SARS-CoV-2 spike A67V for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike A67V (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

A67V

Promoter

CAG

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 N969K (AAA)

Plasmid

#212485

Purpose

Encodes SARS-CoV-2 spike N969K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N969K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N969K

Promoter

CAG

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 F456L (TTA)

Plasmid

#212536

Purpose

Encodes SARS-CoV-2 spike F456L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F456L (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F456L

Promoter

CAG

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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