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pJUMP18-RBS-pET_R

Plasmid

#127013

Purpose

Basic Part R- RBS; pET vector Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part RBS-pET_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0034_R

Plasmid

#127012

Purpose

Basic Part R- RBS; B0034m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0034m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0033_R

Plasmid

#127011

Purpose

Basic Part R- RBS; B0033m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0033m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0032_R

Plasmid

#127010

Purpose

Basic Part R- RBS; B0032m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0032m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pmer_P

Plasmid

#127008

Purpose

Basic Part P- Promoter (non-constitutive); Pmer; metal sensitive; controlled by merR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pmer_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pars_P

Plasmid

#127007

Purpose

Basic Part P- Promoter (non-constitutive); Pars; metal sensitive; controlled by arsR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pars_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pci_P

Plasmid

#127006

Purpose

Basic Part P- Promoter (non-constitutive); Pci; controlled by cI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pci_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-Ptet_P

Plasmid

#127005

Purpose

Basic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Ptet_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23119s_P

Plasmid

#127001

Purpose

Basic Part P- Promoter (constitutive); J23119 (1bp short on 3').

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23119s_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23106_P

Plasmid

#126999

Purpose

Basic Part P- Promoter (constitutive); J23106.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23106_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23103_P

Plasmid

#126998

Purpose

Basic Part P- Promoter (constitutive); J23103.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23103_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF2NBAA

Plasmid

#107873

Purpose

Bacterial expression of FGF2 minimum export mutant-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-minimal export

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

C77A, C95A, K128Q, R129Q, K134Q

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9-GFP T119D

Plasmid

#114668

Purpose

Expresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Promoter

ATG9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE.zeo/hKGA.S286A

Plasmid

#110357

Purpose

Mammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tag

Depositor

Sandra Martha Gomes Dias

Insert

KGA glutaminase (GLS Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Mutation

S286A

Promoter

gag

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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PP245

Plasmid

#78965

Purpose

Promoter: GAP, minimal Promoter: AOX1. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*-(ΔLF)

Plasmid

#80045

Purpose

Expression of Vpr binding mutant EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Mutation

Vpr binding site LF45 in p6 mutated

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔI)

Plasmid

#80048

Purpose

Expression of EPN-01 assembly mutant L33R

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(L33R)

Tags

Myc

Expression

Mammalian

Mutation

L33R

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔL1+L2)

Plasmid

#80051

Purpose

Expression of EPN-01 mutated in both L domains

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaPTAP/deltaYP)

Tags

Myc

Expression

Mammalian

Mutation

PTAP10AAAA, YP37AA

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔI)

Plasmid

#80041

Purpose

Expression of EPN-01 assembly mutant L33R

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Mutation

changed Leu33 in I3-01 to Ala

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PP258

Plasmid

#78962

Purpose

Promoter: GAP6, minimal Promoter: mCYC. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP310

Plasmid

#78979

Purpose

245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

GFP
RFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP152

Plasmid

#78986

Purpose

Supplemental Figure 4. Promoter long ppTEF1. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP153

Plasmid

#78997

Purpose

Supplemental Figure 4. Promoter GAP7. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP259

Plasmid

#78961

Purpose

Promoter: scTEF1, minimal Promoter: mCYC. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP362

Plasmid

#78981

Purpose

255R. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses GFP and RFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

GFP
RFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode49

Plasmid

#83726

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode49

Use

Retroviral

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode42

Plasmid

#83719

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode42

Use

Retroviral

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode18

Plasmid

#83695

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode18

Use

Retroviral

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode92

Plasmid

#83769

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode92

Use

Retroviral

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode90

Plasmid

#83767

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode90

Use

Retroviral

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge