We narrowed to 22,191 results for: his

1. pJCC_084 Cas12i1 D647A

   Plasmid

   #171671

   Purpose

   For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1 D647A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed aspartate 647 to alanine

   Promoter

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPD187 Tri-AND Gate, 2 repeats

   Plasmid

   #138940

   Purpose

   EYFP reporter vector for the Logic 37 AND 43 AND 158 2 repeats promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   Logic 37 AND 43 AND 158 2 repeats
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Logic 37 AND 43 AND 158 2 repeats

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPD186 Tri-AND Gate, 1 repeat

   Plasmid

   #138939

   Purpose

   EYFP reporter vector for the Logic 37 AND 43 AND 158 1 repeat promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   Logic 37 AND 43 AND 158 1 repeat
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Logic 37 AND 43 AND 158 1 repeat

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPD129 AND Gate, 4 repeats

   Plasmid

   #138938

   Purpose

   EYFP reporter vector for the Logic 37 AND 158 NOT 43 4 repeats promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   Logic 37 AND 158 NOT 43 4 repeats
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Logic 37 AND 158 NOT 43 4 repeats

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPD127 AND Gate, 2 repeats

   Plasmid

   #138936

   Purpose

   EYFP reporter vector for the Logic 37 AND 158 NOT 43 2 repeats promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   Logic 37 AND 158 NOT 43 2 repeats
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Logic 37 AND 158 NOT 43 2 repeats

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPD126 AND Gate, 1 repeat

   Plasmid

   #138935

   Purpose

   EYFP reporter vector for the Logic 37 AND 158 NOT 43 1 repeat promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   Logic 37 AND 158 NOT 43 1 repeat
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Logic 37 AND 158 NOT 43 1 repeat

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPD270 ZF43x6-Compact(overlapping ZF37)_EYFP

   Plasmid

   #138934

   Purpose

   EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact(overlapping ZF37)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF43x6-Compact(overlapping ZF37)

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPD173 ZF43(AAAA)i

   Plasmid

   #138833

   Purpose

   Constitutive expression of ZF43(AAAA)i under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43(AAAA)i
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   R6A, R15A, R43A, R71A

   Promoter

   CMV

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPD389 VPR-ZF43(AAAA)

   Plasmid

   #138826

   Purpose

   Constitutive expression of VPR-ZF43(AAAA) under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VPR-ZF43(AAAA)
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   R6A, R15A, R43A, R71A

   Promoter

   CMV

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPD388 VP64-ZF43(AAAA)

    Plasmid

    #138822

    Purpose

    Constitutive expression of VP64-ZF43(AAAA) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP64-ZF43(AAAA)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R6A, R15A, R43A, R71A

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPD113 VP16-ZF43(AAAA)

    Plasmid

    #138818

    Purpose

    Constitutive expression of VP16-ZF43(AAAA) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43(AAAA)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R6A, R15A, R43A, R71A

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEL1514

    Plasmid

    #155147

    Purpose

    Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araBAD

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBad-CA TAG186

    Plasmid

    #105837

    Purpose

    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 186

    Promoter

    pBad/arabinose

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBad-CAts TAG97

    Plasmid

    #105668

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 97

    Promoter

    pBad/arabinose

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMYA5/pDEST17

    Plasmid

    #98115

    Purpose

    Express CMYA5 (aa 4039 – 4069) with a N-terminal 6x-His tag in bacteria

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Syn3D71

    Plasmid

    #14680

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector; aa 1-71 …

    Promoter

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

17. Syn3D96

    Plasmid

    #14681

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector; aa 1-96 …

    Promoter

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

18. Syn3D119

    Plasmid

    #14682

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector; aa 1-119…

    Promoter

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

19. Syn3F35A

    Plasmid

    #13402

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. F35 of s…

    Promoter

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

20. Syn3F36A

    Plasmid

    #13403

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. F36 of s…

    Promoter

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pLN432 (FuGW-G14p-mKate2)

    Plasmid

    #105187

    Purpose

    Module 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G14p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28b(+)-HP0377

    Plasmid

    #39334

    Depositor

    SeWon Suh

    Insert

    oxidoreductase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pPMS-2008

    Plasmid

    #232288

    Purpose

    Protein Expression

    Depositor

    Jeremy Nathans

    Type

    Empty backbone

    Use

    Tags

    8X His-Avi-1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Human SHIP2

    Plasmid

    #124645

    Purpose

    Expresses HIS tagged human SHIP2 in COS-7/HEK cells

    Depositor

    Christophe Erneux

    Article

    Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-5

    Insert

    INPPL1 (INPPL1 Human)
    

    Use

    Tags

    His Xpress (N-terminal)

    Expression

    Mammalian

    Mutation

    coding sequence starts at amino acid 18 as calcul…

    Promoter

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MtPT4

    Plasmid

    #117096

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    MtPT4
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET29b-Cwc25

    Plasmid

    #111341

    Purpose

    his tagged Cwc25

    Depositor

    Christine Guthrie

    Insert

    CWC25
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28B-Cwc25

    Plasmid

    #111340

    Purpose

    his tagged Cwc25

    Depositor

    Christine Guthrie

    Insert

    CWC25
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDB364

    Plasmid

    #110275

    Purpose

    N-terminal 10-His DsbC TEV-peptide fusion

    Depositor

    Christopher Bahl

    Article

    Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    10-His, TEV cleavage peptide, and mature DsbC (i.…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSP1E3D1

    Plasmid

    #20066

    Purpose

    Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

    Insert

    MSP1E3D1
    

    Use

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeats of …

    Promoter

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLexM-SLC3A2

    Plasmid

    #197994

    Purpose

    Expresses SLC3A2 as an N-terminal His tagged construct in mammalian cells

    Depositor

    Simon Newstead

    Article

    Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

    Insert

    SLC3A2 (SLC3A2 Human)
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Please see depositor comments below

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1 myc-RNF114

    Plasmid

    #58295

    Purpose

    mammalian expression of myc tagged RNF114

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

32. LM627

    Plasmid

    #191551

    Purpose

    LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Use

    Tags

    SNAC-tag, HIS-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCRCT

    Plasmid

    #60621

    Purpose

    Plasmid encoding iCas9, tracrRNA and crRNAs

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNA3.1-hMcl-1

    Plasmid

    #25375

    Depositor

    Roger Davis

    Article

    Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

    Insert

    MCL1 (MCL1 Human)
    

    Use

    Tags

    V5 and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MSCV-PIG-Cre

    Plasmid

    #50935

    Purpose

    retroviral expression of Cre recombinase

    Depositor

    Pier Pandolfi

    Article

    Chen et al Nature. 2005 Aug 4;436(7051):725-30.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Retroviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-28c(+)-YTHDF3

    Plasmid

    #102276

    Purpose

    For bacterial expression of His-tagged human YTHDF3

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    6X HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCT310

    Plasmid

    #111140

    Purpose

    Bacterial expression of 6His-dCas9-3XFlag

    Depositor

    Robert Tjian

    Article

    Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

    Insert

    dCas9
    

    Use

    Tags

    3X Flag and 6X His

    Expression

    Bacterial

    Mutation

    D10A - H840A

    Promoter

    T7

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a/Cas9-Cys

    Plasmid

    #53261

    Purpose

    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

    Depositor

    Hyongbum Kim

    Article

    Ramakrishna et al Genome Res. 2014 Apr 2.

    Insert

    Cas9-Cys
    

    Use

    Tags

    6X His tag, HA epitope, and NLS

    Expression

    Bacterial

    Mutation

    Additional Cysteine is added to the C-terminal re…

    Promoter

    T7 Promoter

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pETM33_SUFU_Sufu

    Plasmid

    #178488

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    SUFU_Sufu (SUFU Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEThT-CAHS3

    Plasmid

    #90028

    Purpose

    To express His-tagged CAHS3 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge