We narrowed to 3,483 results for: mpo

1. Burden Monitor lambda version

   Plasmid

   #66073

   Purpose

   The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   GFP
   

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Plasmid

   #92390

   Purpose

   expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pST1374-N-NLS-flag-linker-Cas9-H840A

   Plasmid

   #51129

   Purpose

   Cas9 nickase optimized for nuclear import.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Insert

   Cas9 nickase
   

   Use

   CRISPR

   Tags

   Flag and NLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Actin Binding Domain Tensionless Control

   Plasmid

   #215371

   Purpose

   Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   Actin Binding Domain Tensionless Control
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. YFP-SspBx6

   Plasmid

   #103778

   Purpose

   expression of iPOLYMER component in mammalian cells

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   YFP-SspBx6
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-OSF-VPS15

   Plasmid

   #99326

   Purpose

   Component for mammalian expression of recombinant PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

   Insert

   VPS15 (PIK3R4 Human)
   

   Tags

   Strep-Strep-Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

7. LiPOP1

   Plasmid

   #168264

   Purpose

   A single-component optogenetic tool for photoswitchable necroptosis

   Depositor

   Yubin Zhou

   Article

   He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

   Insert

   MLKL(1-125) (MLKL Human)
   

   Tags

   mCherry, CRY2-PHR

   Expression

   Mammalian

   Mutation

   H15A, K16A, R17A

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)

   Plasmid

   #185802

   Purpose

   one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   cmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag
   

   Expression

   Mammalian

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Burden Monitor phi80 version

   Plasmid

   #66074

   Purpose

   The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   GFP
   

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. HB501: pMVP (L1-L4) RIP

    Plasmid

    #121688

    Purpose

    pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Rat Ins1 promoter (RIP; β-cell specific)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-GBP1-10gly-Gal4DBD

    Plasmid

    #49438

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-Gal4 DNA binding domain fusion protein
    

    Expression

    Mammalian

    Mutation

    C98S (to increase protein solubility), Q3D

    Promoter

    CAG

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-N-CretrcintG

    Plasmid

    #69572

    Purpose

    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

    Insert

    kozak consensus - N-CretrcintG
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-C-CreintG

    Plasmid

    #69573

    Purpose

    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

    Insert

    kozak consensus - C-CreintG
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mRFP-LAP2β

    Plasmid

    #228029

    Purpose

    lentiviral construct for mRFP-LAP2β expression

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    LAP2b (Tmpo Rat)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LD101: pMVP (L3-L2) eCFP + WPRE

    Plasmid

    #121775

    Purpose

    pMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eCFP + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. mNeonGreen-Sec24D

    Plasmid

    #226581

    Purpose

    Encodes Sec24D protein labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

    Insert

    Sec24D (Sec24 homolog D) (SEC24D Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. P447-FBP_19

    Plasmid

    #139710

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsive

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA

    Plasmid

    #121750

    Purpose

    pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    3x HA epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

    Plasmid

    #171795

    Purpose

    pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CFP-FRBx5-His

    Plasmid

    #103784

    Purpose

    expresses His-tagged iPOLYMER component in E. coli for protein purification

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    CFP-FRBx5-His
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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