We narrowed to 4,337 results for: Pet2

1. pJK326

   Plasmid

   #71557

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-368 to aspartate

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK325

   Plasmid

   #71556

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-367 to aspartate

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK500

   Plasmid

   #72245

   Purpose

   Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and endogenous

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK345

   Plasmid

   #71559

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   deletes alanine-367 and alanine-368

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK344

   Plasmid

   #71558

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   deletes alanine-367 (same as deleting alanine-368)

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK624

   Plasmid

   #72539

   Purpose

   Produces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK625

   Plasmid

   #72541

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK392

   Plasmid

   #70236

   Purpose

   Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE-H40N

   Depositor

   T. J. Kappock

   Article

   Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Expression

   Bacterial

   Mutation

   His40Asn

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. 4QQI

   Plasmid

   #61949

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ANKRA:JMC023-D07:C211743 (ANKRA2 Human)
   

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   March 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. 2MJ8

    Plasmid

    #61956

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CDYL2 (CDYL2 Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. 4LG6

    Plasmid

    #60093

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ANKRA:JMC023-D07:C211743 (ANKRA2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. L3MBTL1 (3P8H)

    Plasmid

    #28265

    Depositor

    Cheryl Arrowsmith

    Insert

    L3MBTL1 (L3MBTL1 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 200-530.

    Available Since

    April 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

13. CHKB (2IG7)

    Plasmid

    #25516

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CHKB (CHKB Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 31, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. ARL10B

    Plasmid

    #25522

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARL10B (ARL8A Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. CENTA1 (3LJU)

    Plasmid

    #25340

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CENTA1:K3-F370 (ADAP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids K3-F370

    Available Since

    Aug. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. CENTA1 (3FEH)

    Plasmid

    #25262

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CENTA1:K3-F370 (ADAP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids K3-F370

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. WDR5 (2H9O)

    Plasmid

    #25230

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5_01 (WDR5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. PRRX2-pET

    Plasmid

    #21011

    Depositor

    Corey Largman

    Article

    Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

    Insert

    PRRX2 (Prrx2 Mouse)
    

    Tags

    His and T7-tag

    Expression

    Bacterial

    Mutation

    Missing first 3 N-terminal amino acids

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

19. V48 ELP

    Plasmid

    #68395

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    V48 ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK225

    Plasmid

    #71124

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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