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pCAG-EGFP-N-IFT80

Plasmid

#218731

Purpose

Expresses C-terminally EGFP-tagged IFT80 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

IFT80 (IFT80 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAIP-hXRCC1wt-eGFP

Plasmid

#206032

Purpose

Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

Depositor

Primo Schaer

Publication

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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PB-CAG-ChR2-YFP

Plasmid

#204345

Purpose

PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

Depositor

Ivo Lieberam

Publication

Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

Insert

ChR2-YFP

Tags

Yellow fluorescent protein

Expression

Mammalian

Mutation

C-terminus (aa315-737) is deleted - PMID: 1461559…

Promoter

CAG

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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BA31_pU.CAG.SaCas9-D10A.rBGpA

Plasmid

#199251

Purpose

Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

Depositor

Manuel Goncalves

Publication

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SaCas9.D10A nickase

Use

CRISPR

Tags

3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-dDIO-MCS--4x6T

Plasmid

#196422

Purpose

Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Publication

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Dre/rox; astrocyte-selective

Expression

Mammalian

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-flex-lck-smV5

Plasmid

#196423

Purpose

Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

Depositor

Stanley Thomas Carmichael

Publication

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smV5

Use

AAV and Cre/Lox

Tags

Lck

Expression

Mammalian

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-hfYFP

Plasmid

#191201

Purpose

Constitutive CAG-driven expression of hfYFP in animals using AAV

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Use

AAV

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CAG

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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UPB-duodenum (UPB6)

Plasmid

#180785

Purpose

Unique Projection Barcode (UPB6)-Duodenum for Projection-seq

Depositor

Rui Chang

Publication

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

hM4D(Gi) (partial )

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Kv-ArcLight-ST

Plasmid

#182319

Purpose

Soma-targeted fluorescent voltage indicator with left-shifted voltage-dependency

Depositor

Rafael Yuste

Publication

Bando et al Nat Commun. 2021 Dec 10;12(1):7229. doi:

Insert

Kv-ArcLight-ST

Expression

Mammalian

Mutation

Mutation in an amino acid Q217E of ArcLight-MT

Promoter

CAG

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pD2529-CAG-HA-mouse GARP

Plasmid

#180258

Purpose

Mouse full length GARP expression

Depositor

Timothy Springer

Publication

Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

Insert

GARP (Lrrc32 Mouse)

Tags

HA

Expression

Mammalian

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CIBN-EGFP-KRasCT

Plasmid

#178527

Purpose

Expresses CIBN-EGFP-KRasCT in mammalian cells

Depositor

Kazuhiro Aoki

Publication

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CIBN-EGFP-KRasCT

Expression

Mammalian

Promoter

CAG

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

Plasmid

#175177

Purpose

Cre-dependent GCaMP6s and mBeRFP expression

Depositor

Bernardo Sabatini

Publication

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

GCaMP6s

Use

AAV

Tags

P2A-mBeRFP

Promoter

CAG

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA

Plasmid

#172912

Purpose

Mammalian expression of voltage sensor; soma localization targeting sequence

Depositor

GENIE Project

Publication

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Ace2N-4AA-mNeon-ST A122D

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta2-3xFiBsd

Plasmid

#140964

Purpose

full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Publication

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis-associated gene family, member 2 (Mta2 Mouse)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-G_oT7pol-IRES-BSD

Plasmid

#172369

Purpose

Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

Depositor

Yoav Ben-Simon, Peter Jonas

Publication

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

oT7pol + BSD

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8_Flag2-Blast

Plasmid

#175038

Purpose

Mammalian expression of full length mMPP8 with C-terminal Flag2 tag

Depositor

Kristian Helin

Publication

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Promoter

CAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-SNAPf-CCR5TC

Plasmid

#135537

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to SNAPf, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Publication

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

SNAPf

Expression

Mammalian

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-TWIN-STREP-FLAG-NPRL2

Plasmid

#164985

Purpose

NPRL2 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Publication

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

NPRL2 (NPRL2 Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

Plasmid

#159630

Purpose

AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

Depositor

Andrew Allen, Ross Bathgate

Publication

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

AAV

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Promoter

DCA (same as CAG promoter)

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-eGFP-Z1

Plasmid

#135046

Purpose

Enhanced Green fluorescent protein under constitutive CAG Promoter for mammalian expression in minimal backbone plasmid

Depositor

Jordan Green

Publication

Yang et al PLoS One. 2026 Jul 23;21(7):e0352468. do

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG Promoter

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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