We narrowed to 15,088 results for: pET

1. PET-B-eSpCas9-NLS-6xHis

   Plasmid

   #207383

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, amino acids 1005-1013 repl…

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet RagA(D130N)-RagC

   Plasmid

   #99642

   Purpose

   Bacterial expression of RagA(D130N)-RagC

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D130N

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-KIX (586-672)

   Plasmid

   #99336

   Purpose

   Bacterial expression of mouse CBP KIX domain

   Depositor

   Peter Wright

   Article

   Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

   Insert

   CBP KIX (586-672) (Crebbp Mouse)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-TGFα-GSY-SPM

   Plasmid

   #163615

   Purpose

   Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

   Depositor

   Mark Howarth

   Article

   Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

   Insert

   TGFa-GSY-SPM (TGFA Synthetic, Human)
   

   Tags

   C-tag and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-His6-Ubc9-K14R

   Plasmid

   #185759

   Purpose

   Bacterial expression of His6-Ubc9-K14R

   Depositor

   Jeffrey Bode

   Article

   Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   K14R

   Promoter

   T7

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET28a-cpLumiLuc-Rapamycin sensor

   Plasmid

   #186292

   Purpose

   Expression Rapamycin sensor in e coli

   Depositor

   Kirill Alexandrov

   Article

   Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

   Insert

   cpLumiLuc-Rapamycin sensor
   

   Tags

   HisTag

   Expression

   Bacterial

   Available Since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet RagA(T21N)-RagC

   Plasmid

   #99644

   Purpose

   Bacterial expression of RagA(T21N)-RagC

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   T21N

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-6His-SUMO-Nsp14

   Plasmid

   #154956

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
   

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAAV-TRE-YPet-WPRE

   Plasmid

   #193342

   Purpose

   AAV-mediated expression of YPet under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   YPet
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SENP5 (catalytic domain)

    Plasmid

    #16358

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP5 catalytic domain (SENP5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 567 to 755 catalytic domain of human S…

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pET23-PBD(65-150)-N-Cys

    Plasmid

    #13722

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    PBD(65-150)-N-Cys (PAK1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Met-Cys-Gly-PBD(65-150)-

    Available Since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pET22b-mEB2-eGFP-6xHis

    Plasmid

    #130982

    Purpose

    Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB2-GFP-6xHis (Mapre2 Mouse)
    

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-MBP-TEV-PWWP_DNMT3A

    Plasmid

    #186970

    Purpose

    Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    DNMT3A PWWP domain (DNMT3A Synthetic, Human)
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    PWWP domain (residues 278–427)

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-FLAG-SpCas9-HF1

    Plasmid

    #126770

    Purpose

    Expression of increased fidelity SpCas9-HF1 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET51b(+)_LAMA-G97 (for GFP)

    Plasmid

    #130715

    Purpose

    Plasmid encoding the bacterial expression of LAMA-G97 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-G97
    

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET151-D-TOPO-PARP14-SΔK

    Plasmid

    #216658

    Purpose

    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

    Depositor

    Herwig Schüler

    Article

    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

    Insert

    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    Deletion starting from Q1390 to D1448 and substit…

    Promoter

    T7

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet RagA-RagC(D181N)

    Plasmid

    #99643

    Purpose

    Bacterial expression of RagA-RagC(D181N)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D181N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM10 with rat FL Annexin A11

    Plasmid

    #164496

    Purpose

    For bacterial expression of rat His-tagged FL Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat FL Annexin A11 (Anxa11 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET30a-His6-ABP-LOXCATmut

    Plasmid

    #139972

    Purpose

    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-his6-HA3-HALO

    Plasmid

    #188943

    Purpose

    T7 expression vector for making his6- and HA3-tagged HALO protein

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    his6-HA3-HALO
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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