We narrowed to 17,254 results for: tes

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p3E-DrCav1

Plasmid

#194290

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin1 (cav1.S Frog)

Use

Multisite gateway entry vector

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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SITS-MmCav1-EGFP-APEX2

Plasmid

#170525

Purpose

Vector expression of MmCav1-EGFP-APEX2 in Leishmania cell free system. Parton lab clone JEJ

Depositor

Rob Parton

Publication

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Caveolin1 (Cav1 Mouse)

Use

Synthetic Biology

Tags

APEX2

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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MmCavin4-APEX2-P2A-mKate2

Plasmid

#170524

Purpose

Vector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJF

Depositor

Rob Parton

Publication

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cavin4 (Cavin4 Mouse)

Tags

APEX2-P2A-mKate2

Expression

Mammalian

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-LactC2

Plasmid

#109499

Purpose

Muscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-LactC2

Use

Zebrafish plasmids

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCascade-IL1RNcrRNA

Plasmid

#126493

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RN

Depositor

Charles Gersbach

Publication

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo IL1RN cr03

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCascade-HBE1crRNA

Plasmid

#126494

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1

Depositor

Charles Gersbach

Publication

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo HBE1 cr06

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAPcrRNA_Eco

Plasmid

#106276

Purpose

E.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoter

Depositor

Charles Gersbach

Publication

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSPIN-GG

Plasmid

#251750

Purpose

Single-plasmid V. cholerae CAST encoding proteins, crRNA, and donor DNA. Entry vector: GFP dropout; BbsI spacer, BsaI promoter swap, SAPI donor cloning; pSC101* backbone.

Depositor

Thomas Gorochowski

Publication

Irvine et al Methods Mol Biol. 2026;3041:33-45. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pJW2332

Plasmid

#253923

Purpose

linker::mNeonGreen::3xFLAG::linker with unique crRNA sites

Depositor

Jordan Ward

Publication

Ragle et al bioRxiv [Preprint]. 2025 May 14:2025.05.

Insert

12aa linker::mNeonGreen::TEV::3xFLAG::12aa linker

Expression

Worm

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pJW2333

Plasmid

#253924

Purpose

linker::mNeonGreen::AID*::3xFLAG::linker with unique crRNA sites

Depositor

Jordan Ward

Publication

Ragle et al bioRxiv [Preprint]. 2025 May 14:2025.05.

Insert

12aa linker::mNeonGreen::TEV::AID*::3xFLAG::12aa linker

Expression

Worm

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pJW2471

Plasmid

#253925

Purpose

linker::mScarlet (GLO)::3xHA::linker for re-editing knock-in strains

Depositor

Jordan Ward

Publication

Ragle et al bioRxiv [Preprint]. 2025 May 14:2025.05.

Insert

12aa linker::mScarlet (GLO)::3xHA::12aa linker

Expression

Worm

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pJW2558

Plasmid

#253926

Purpose

linker::mScarlet (GLO)::2xOLLAS::linker for re-editing knock-in strains

Depositor

Jordan Ward

Publication

Ragle et al bioRxiv [Preprint]. 2025 May 14:2025.05.

Insert

12aa linker::mScarlet (GLO)::2xOLLAS::12aa linker

Expression

Worm

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pFastBac-M13-8xHis-ZZ-LTLT-HOOK3

Plasmid

#222287

Purpose

Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease).

Depositor

Anne Straube

Publication

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Insert

HOOK3 (HOOK3 Human)

Tags

8 His and ZZ tag

Expression

Insect

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

Plasmid

#222288

Purpose

Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.

Depositor

Anne Straube

Publication

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Insert

KIF1C (KIF1C Human)

Tags

6xHis, GFP, and GST

Expression

Bacterial

Mutation

stalk region only amino acids 642 to 922

Promoter

T7

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook3_FTS_FHIP1B

Plasmid

#222299

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Publication

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK3 (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Tags

StrepII-Psc

Expression

Bacterial

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

Plasmid

#222300

Purpose

Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Publication

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK3[451-718] (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Tags

StrepII-Psc

Expression

Insect

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B

Plasmid

#222301

Purpose

Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Publication

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK3[571-718] (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Tags

StrepII-Psc

Expression

Insect

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINE-GFP

Plasmid

#223706

Purpose

For production of EGFP by E.coli

Depositor

Rob Parton

Publication

Luong et al ACS Nano. 2025 Dec 23;19(50):42079-42096

Insert

EGFP

Expression

Bacterial

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-mCherry

Plasmid

#118753

Purpose

Multisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFF

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

mCherry

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb

Plasmid

#141067

Purpose

Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Synthetic Biology

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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