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Showing: 6281 - 6320 of 8366 results

  2. AICSDP-52: HIST1H2BJ-mEGFP

    Plasmid
    #109121
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ
    Depositor
    Allen Institute for Cell Science
    Insert
    HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109121-plasmid-map-sequence-id-209767

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  3. Hs.KRAS4a

    Plasmid
    #83166
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83166-plasmid-map-sequence-id-158129

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  4. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50464
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote
    Depositor
    Bryan Roth
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50464-plasmid-map-sequence-id-181128

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  5. CAMK2G

    Plasmid
    #38894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CAMK2G (CAMK2G Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. psd44-G12QL

    Plasmid
    #46825
    Purpose
    expression of mutant G protein alpha 12 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA12 (GNA12 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Q231L (the mutation reduces GTPase activity resul…
    Promoter
    PGK
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46825-plasmid-map-sequence-id-410918

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  8. pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50471
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Bryan Roth
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50471-plasmid-map-sequence-id-75491

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  9. pAAV-Ef1a-Flex-Axon-GCaMP7b

    Plasmid
    #135419
    Purpose
    Can be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.
    Depositor
    Rylan Larsen
    Insert
    axon-jGCaMP7b
    Use
    AAV
    Tags
    6xHis and GAP43 palmitoylation domain
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135419-plasmid-map-sequence-id-271053

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  10. pAG426-GAL-Alpha-synuclein-E46K

    Plasmid
    #181723
    Purpose
    Galactose inducible expression of Alpha-synuclein-E46K
    Depositor
    Alejandro Chavez
    Insert
    Alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    E46K
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    181723-plasmid-map-sequence-id-439543

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  11. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid
    #225684
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.
    Depositor
    Ryan Mehl
    Inserts
    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    Use
    Methanomethylophilus alvus
    Tags
    Expression
    Mutation
    CTG125CTT, N166A, V168S, A223C, W239R
    Promoter
    GlnS and Lpp
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    225684-plasmid-map-sequence-id-450701

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  12. AICSDP-117:POLR2A-mEGFP

    Plasmid
    #164500
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A
    Depositor
    Allen Institute for Cell Science
    Insert
    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164500-plasmid-map-sequence-id-324900

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  13. Hs.KRAS4a G12D

    Plasmid
    #83168
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12D
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83168-plasmid-map-sequence-id-158131

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  14. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50467
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV2 and AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50467-plasmid-map-sequence-id-199198

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  15. MS2_SC2_S_Delta

    Plasmid
    #175950
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Delta (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175950-plasmid-map-sequence-id-345521

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  16. huntingtin_FL_NbioCflag

    Plasmid
    #194767
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    July 27, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194767-plasmid-map-sequence-id-402615

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  19. Hs.NRAS G12V

    Plasmid
    #83174
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12V mutation
    Depositor
    Dominic Esposito
    Insert
    neuroblastoma RAS viral oncogene homolog (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12V
    Promoter
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    83174-plasmid-map-sequence-id-158137

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  20. 6xO/S luc

    Plasmid
    #69445
    Purpose
    detection/assessment of OCT4 and SOX2 transcription factor activity
    Depositor
    Lisa Dailey
    Insert
    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    69445-plasmid-map-sequence-id-259015

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  21. SARS-CoV-2 Spike-d18

    Plasmid
    #164583
    Purpose
    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.
    Depositor
    Jason Sheltzer
    Insert
    SARS-CoV-2 Spike (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1256-1273
    Promoter
    EF1a
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164583-plasmid-map-sequence-id-322362

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  22. ALDH18A1

    Plasmid
    #38947
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ALDH18A1 (ALDH18A1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. DDX3X in pENTR

    Plasmid
    #216572
    Purpose
    gateway cloning donor vector containing DDX3X
    Depositor
    Douglas Allan, Christopher Loewen
    Insert
    DDX3X (DDX3X Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216572-plasmid-map-sequence-id-426667

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  24. PE-SUMO CRYAB

    Plasmid
    #217670
    Purpose
    For expression of SUMO tagged human alpha B crystallin
    Depositor
    Larry David
    Insert
    CRYAB (CRYAB Human)
    Use
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217670-plasmid-map-sequence-id-434869

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  25. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid
    #66708
    Purpose
    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
    Depositor
    Charles Gersbach
    Insert
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Expression
    Mutation
    D10A and H840A
    Promoter
    hUbC
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66708-plasmid-map-sequence-id-449286

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  26. PE-SUMO CRYBB1

    Plasmid
    #217675
    Purpose
    For expression of SUMO tagged human beta B1 crystallin
    Depositor
    Larry David
    Insert
    CRYBB1 (CRYBB1 Human)
    Use
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217675-plasmid-map-sequence-id-434867

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  27. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  30. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid
    #159912
    Purpose
    Mutagenesis of Kcnd3
    Depositor
    Larry Zweifel
    Insert
    Kcnd3 (Kcnd3 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159912-plasmid-map-sequence-id-317319

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  31. AICSDP-69: NPM1-mTagRFP-T

    Plasmid
    #124608
    Purpose
    Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1
    Depositor
    Allen Institute for Cell Science
    Insert
    NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    124608-plasmid-map-sequence-id-244304

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  33. Hs.KRAS4b G12C

    Plasmid
    #83130
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12C
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83130-plasmid-map-sequence-id-158093

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  34. pLPS-hSox2-gfp

    Plasmid
    #49390
    Purpose
    mammalian expression of human Sox2 fused to EGFP
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available since
    Nov. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    49390-plasmid-map-sequence-id-354145

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  35. pAAV-TREtight-mTagBFP2-N2cG

    Plasmid
    #192838
    Purpose
    plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA
    Depositor
    Ian Wickersham
    Insert
    mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192838-plasmid-map-sequence-id-378336

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  36. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid
    #218710
    Purpose
    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218710-plasmid-map-sequence-id-433357

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  37. PTPN11 in pENTR

    Plasmid
    #216604
    Purpose
    gateway cloning donor vector containing PTPN11
    Depositor
    Douglas Allan, Christopher Loewen
    Insert
    PTPN11 (PTPN11 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216604-plasmid-map-sequence-id-427162

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  39. pCDF1-mAtf4-HT-puro

    Plasmid
    #101795
    Purpose
    Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4
    Depositor
    Seiichi Oyadomari
    Insert
    Activating transcription factor 4 (Atf4 Mouse)
    Use
    Lentiviral
    Tags
    Halo tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    101795-plasmid-map-sequence-id-194514

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  40. Hs.KRAS4b Q61H

    Plasmid
    #83140
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61H
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83140-plasmid-map-sequence-id-158103

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Showing: 6281 - 6320 of 8366 results