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pCI Flag Smad3 LC WT

Plasmid

#26996

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_AAVS1D

Plasmid

#183340

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1D

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1C

Plasmid

#183339

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1C

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC4

Plasmid

#183336

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC4

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC3

Plasmid

#183335

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC3

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC2

Plasmid

#183334

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC2

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC1

Plasmid

#183333

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC1

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BRD4

Plasmid

#183331

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRD4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRD4

Use

Lentiviral

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG013

Plasmid

#231334

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid with FRT site. L1-p15A-Gm-FRT-ELT4; GG assembly from pNDGG021, pOGG009, pNDGG006, pNDGG009, pOGG014. NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pQGG001

Plasmid

#231313

Purpose

Vector part for BEVA; BEVA2.0 Position 4 I-SceI recognition/cut site, SpR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 4 I-SceI recognition/cut site, SpR.

Expression

Bacterial

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_2-3_005_Promoter+5'UTR_Placeholder

Plasmid

#235870

Purpose

Encodes a sfGFP cassette in the promoter+5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

Promoter + 5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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CARMEN

Plasmid

#240464

Purpose

Compartment-specific measurements of Ca²⁺ dynamics by intensiometric Ca²⁺ biosensors targeted to mitochondria, cytosol and ER

Depositor

Roland Malli

Article

Lischnig et al Br J Pharmacol. 2025 Oct 3. doi: 10.1111

Insert

CARMEN

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBR322-sgRNA-pvc18-20-Pdp1_NTD-Cre

Plasmid

#243748

Purpose

PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA - Cre loaded into the PVC tube via Pdp1 (NTD)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

vegfa sgRNA-pvc18-20-Pdp1_NTD-Cre

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_pvc10-ZFD

Plasmid

#243743

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with C half of a zinc finger deaminase (ZFD) on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_pvc10-ZFD

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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GST-Thrombin-Munc13-1 MUN domain (aa859-1516 delta1408-1452 EF)

Plasmid

#243818

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

GST

Expression

Bacterial

Mutation

1408-1452 --> EF

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-FGD3

Plasmid

#242832

Purpose

Encodes human FGD3 and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

FGD3 (FGD3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-ARHGEF6

Plasmid

#242830

Purpose

Encodes human ARHGEF6 and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

ARHGEF6 (ARHGEF6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-eGFP

Plasmid

#242758

Purpose

Expresses eGFP fluorophore with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Article

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-EGFP

Expression

Bacterial

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG052

Plasmid

#231321

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG010, pOGG012, and pOGG014, SpR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1369

Plasmid

#239828

Purpose

WT OrufIscB mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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