We narrowed to 5,328 results for: pcdna3.1

1. miniCMV-iRFP670-tDeg

   Plasmid

   #185403

   Purpose

   iRFP670-tDeg fluorogenic protein

   Depositor

   Jian-Hui Jiang

   Article

   Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

   Insert

   iRFP670-tDeg
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. VinculinTS-A50I/I997A

   Plasmid

   #111829

   Purpose

   Vinculin tension sensor with point mutations affecting ability to bind talin and actin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A)
   

   Expression

   Mammalian

   Mutation

   changed vinculin alanine 50 to isoleucine and iso…

   Promoter

   CMV

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cenp-B INCENP GFP

   Plasmid

   #45238

   Purpose

   Target INCENP to centromeres via full length CENP-B

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B, INCENP-Δcen, GFP
   

   Tags

   GFP and full length CENP-B

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOVC1_TtCarH-CBD_X

   Plasmid

   #131179

   Purpose

   Expresses TtCarH-CBD (Thermus termophilus) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   TtCarH-CBD
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOVC1_MxCarH-CBD_X

   Plasmid

   #131178

   Purpose

   Expresses MxCarH-CBD (Myxococcus xanthus) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   MxCarH-CBD
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVH607-Tier1-PCREm4-Nluc

   Plasmid

   #169594

   Purpose

   Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Expression

   Mammalian

   Promoter

   PCREm4

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mito-LSSmGFP

   Plasmid

   #186536

   Purpose

   Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Tags

   Human COX8a first 29 amino acids

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVH231-Tier1-PmPGK1-mTagBFP2

   Plasmid

   #169520

   Purpose

   Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven monomeric Tag blue fluorescent protein 2
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. tCRMod-GGSGGS9

   Plasmid

   #111762

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS9-mRuby2 fusion
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ER2-mGreenLantern

    Plasmid

    #186548

    Purpose

    Localization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    mGreenLantern
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER2-LSSmGFP

    Plasmid

    #186538

    Purpose

    Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPD005 pcDNA Golden Gate

    Plasmid

    #138749

    Purpose

    Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-ALFA-tag-Gal4-VP16

    Plasmid

    #199465

    Purpose

    Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

    Insert

    Gal4-ALFA-tag-VP16
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LacI INCENP

    Plasmid

    #45236

    Purpose

    Target INCENP to lac operator sequence

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    LacI, INCENP-Δcen, mCherry (lacI )
    

    Tags

    LacI (lac repressor protein) and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    t7

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Fra-1

    Plasmid

    #187906

    Purpose

    Expresses Fra-1 in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    Fra1 (Fosl1 Rat)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TR therapy reporter

    Plasmid

    #179083

    Purpose

    Fluorescent reporter for alternative splicing

    Depositor

    Thurman Wheeler

    Article

    Hu et al Nat Commun. 2018 Dec 7;9(1):5227. doi: 1

    Inserts

    DsRed1
    EGFP
    

    Expression

    Mammalian

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVH233-Tier1-PmPGK1-eYFP

    Plasmid

    #169522

    Purpose

    Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven enhanced yellow fluorescent protein
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA_RFP-SZ2

    Plasmid

    #124936

    Purpose

    Expression of RFP-SZ2

    Depositor

    Benjamin Schuster, Daniel Hammer, Matthew Good

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RFP-SYNZIP2
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    SZ2 domain

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEQ1007

    Plasmid

    #83938

    Purpose

    express human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cells

    Depositor

    Adam Geballe

    Article

    Hakki et al J Virol. 2006 Dec;80(23):11817-26. Epub

    Insert

    IRS1
    

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    R833L in IRS1 (please see depositor comment)

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZ143-pcDNA3-BvCas12b

    Plasmid

    #122445

    Purpose

    Expresses BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BvCas12b-NLS-3xHA
    

    Tags

    3XHA

    Expression

    Mammalian

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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