We narrowed to 12,478 results for: BASE;

1. Pink Flamindo

   Plasmid

   #102356

   Purpose

   biosensor for cAMP in mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

   Insert

   Pink Flamindo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-LMNA-gRNA1

   Plasmid

   #122507

   Purpose

   expresses WT spCas9 and a chimeric gRNA targeting human LMNA

   Depositor

   Graham Dellaire

   Article

   Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

   Insert

   LMNA-gRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

   Plasmid

   #185914

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEIGW roGFP2-Orp1

   Plasmid

   #64993

   Purpose

   Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Lentiviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCP-RecT

   Plasmid

   #183827

   Purpose

   pLenti-EF1A-MCP-EXTEN-RecT-NLS

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   MCP-RecT_NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOSCAR

   Plasmid

   #29639

   Depositor

   David_L Andrews, Scott Gold

   Article

   Paz et al Fungal Genet Biol. 2011 Jul;48(7):677-84

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pA-HYG OSCAR

   Plasmid

   #29640

   Depositor

   David_L Andrews, Scott Gold

   Article

   Paz et al Fungal Genet Biol. 2011 Jul;48(7):677-84

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pFA6a-kanMX6

   Plasmid

   #39296

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCpf1b-sp

   Plasmid

   #122188

   Purpose

   Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE30-hPolB

    Plasmid

    #70761

    Purpose

    bacterial expression of human DNA Polymerase beta (POLB)

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    POLB (POLB Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    R333L

    Promoter

    T5/lac operon

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDHt-SK

    Plasmid

    #63615

    Purpose

    The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

    Depositor

    Seogchan Kang

    Article

    Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

    Type

    Empty backbone

    Use

    Fungal expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. TALED_Left-ND1-AD-E1347A

    Plasmid

    #183894

    Purpose

    Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-AAVS1

    Plasmid

    #227272

    Purpose

    Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting AAVS1 (AAVS1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-dTAG-Puro

    Plasmid

    #207727

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. dCas13d-dsRBD

    Plasmid

    #154938

    Purpose

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB_rtTA_BsmBI

    Plasmid

    #126028

    Purpose

    PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-N-Puro-dTAG

    Plasmid

    #207694

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pMK382

    Plasmid

    #214373

    Purpose

    Tet-OsTIR1(F74G) (AAVS1 insertion donor)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Tet-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON 3G promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-BbsI-PITCh

    Plasmid

    #127875

    Purpose

    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXL-BacII-CAG-loxP-STOP-loxP-Venus

    Plasmid

    #170520

    Purpose

    Plasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

    Insert

    CAG-loxP-STOP-loxP-Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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