We narrowed to 5,565 results for: SPI

1. 6xHis-MBP-huTsCas12a

   Plasmid

   #174687

   Purpose

   Expresses 6xHis-MBP tagged TsCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huTsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6x Halo-EGFP- 2x PACT

   Plasmid

   #107265

   Purpose

   6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   6x Halo-EGFP- 2x PACT
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT001

   Plasmid

   #171061

   Purpose

   Plasmid for expressing HIV-1 envelope glycoprotein

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   HIV-1 Env
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDsRed1-N1 RanGAP

   Plasmid

   #13386

   Depositor

   Mary Dasso

   Article

   Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

   Insert

   RanGAP (RANGAP1 Human)
   

   Use

   Tags

   DsRed1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-IRES-GFP-mouse-Caspase-1

   Plasmid

   #183361

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 (Casp1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSSM110: pCMV-HA-NLS-SpuFz1-v2

   Plasmid

   #205277

   Purpose

   Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   HA, NLS(Makkerh)

   Expression

   Mammalian

   Mutation

   C310R/D487K/T513K

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Thy1.1-GFP

   Plasmid

   #131703

   Purpose

   encodes Thy1.1 followed by a P2A site and GFP for enriching pure populations of transfected cells

   Depositor

   Britt Glaunsinger

   Article

   Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

   Insert

   Thy1.1-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB UbC Arrestin-uTEV2-V5; UAS-mCherry

   Plasmid

   #173123

   Purpose

   Expresses uTEV2 and reporter receiver construct in DIPG

   Depositor

   Alice Ting

   Article

   Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

   Insert

   Arrestin-uTEV2-V5; UAS-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRESneo-PA-GFP-alpha Tubulin

   Plasmid

   #12297

   Depositor

   Patricia Wadsworth

   Article

   Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Use

   Tags

   PA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLBH559_Tet-HisCas14a1Locus

    Plasmid

    #112502

    Purpose

    Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

    Plasmid

    #205268

    Purpose

    Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28_mouse-Caspase-1_enzymatic-domain

    Plasmid

    #183385

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (AA130-404) (Casp1 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

    Plasmid

    #205269

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLentiCRISPR-v2 sgATG7

    Plasmid

    #211534

    Purpose

    Deletes ATG7

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgATG7
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs-OLIG2

    Plasmid

    #32933

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    OLIG2 (OLIG2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. ttAtCas12a+int

    Plasmid

    #182384

    Purpose

    Gives very high editing efficiency in barley. Also works in Brassica oleracea

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al bioRxiv 2022.04.28.489853

    Insert

    LbCas12a coding sequence with D156R and Arabidopsis introns
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R + 8 Arabidopsis introns added

    Promoter

    None

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. betaRBPIG

    Plasmid

    #16422

    Depositor

    Juergen Hescheler

    Article

    Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

    Insert

    loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pIREShyg-PA-GFP-alpha Tubulin

    Plasmid

    #12296

    Depositor

    Patricia Wadsworth

    Article

    Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28_human-Caspase-4_enzymatic-domain

    Plasmid

    #183381

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (AA94-377) (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLBH545_Tet-Cas14a1_Locus

    Plasmid

    #112501

    Purpose

    Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge